{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T22:04:40Z","timestamp":1770069880822,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,8,15]]},"abstract":"<jats:p>Protein undernutrition is characterized by growth failure in young growing animals. Current evidence suggests that biosynthesis of insulin-like growth factor (IGF)-I and IGF-binding protein 1 (IGFBP-1) are key control points for nutritional regulation of growth. Here we examined the role of amino acid limitation in regulating the IGFBP-1 expression in the hepatic cell line. Our data show that leucine limitation strongly induces IGFBP-1 without affecting IGF-I and IGF-II expression in human HepG2 cells and in isolated rat hepatocytes. Depletion of arginine, cystine and all essential amino acids leads to induction of IGFBP-1 mRNA and protein expression in a dose-dependent manner. IGFBP-1 expression is significantly induced by leucine concentration in the range of that observed in the blood of rats fed a low-protein diet or in humans affected by kwashiorkor. Moreover, treatment of HepG2 cells with amino acids at a concentration reproducing the amino acid concentration found in portal blood of rats fed a low-protein diet leads to a significantly higher expression of IGFBP-1. These data represent the first demonstration that an amino acid limitation, as occurs during dietary protein deficiency, induces IGFBP-1 expression in hepatic cells. Therefore, amino acids by themselves can play, in concert with hormones, an important role in the control of gene expression.<\/jats:p>","DOI":"10.1042\/bj3340147","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:03:00Z","timestamp":1439244180000},"page":"147-153","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Physiological concentration of amino acids regulates insulin-like-growth-factor-binding protein 1 expression"],"prefix":"10.1042","volume":"334","author":[{"given":"C\u00e9line","family":"JOUSSE","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Nutrition Cellulaire et Mol\u00e9culaire, INRA de Theix, 63122 Saint Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"BRUHAT","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Nutrition Cellulaire et Mol\u00e9culaire, INRA de Theix, 63122 Saint Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"FERRARA","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Nutrition Cellulaire et Mol\u00e9culaire, INRA de Theix, 63122 Saint Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"FAFOURNOUX","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Nutrition Cellulaire et Mol\u00e9culaire, INRA de Theix, 63122 Saint Gen\u00e8s Champanelle, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/334\/1\/147\/632319\/bj3340147.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/334\/1\/147\/632319\/bj3340147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:40:07Z","timestamp":1637714407000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/334\/1\/147\/34386\/Physiological-concentration-of-amino-acids"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3340147","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,8,15]]}}}