{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T05:47:15Z","timestamp":1648964835326},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,8,1]]},"abstract":"<jats:p>1. A granulocyte-rich fraction was isolated from blood of a patient with chronic granulocytic leukaemia and from blood of a normal subject and the cells were disrupted in isotonic sucrose. The nuclei were removed by low-speed centrifugation and the post-nuclear supernatant was fractionated by centrifugation on sucrose density gradients.<\/jats:p>\n               <jats:p>2. The subcellular organelles in the gradients were detected with marker enzymes by the use of highly sensitive assay techniques. Similar results were obtained with granulocytes from both subjects.<\/jats:p>\n               <jats:p>3. Unsaturated vitamin B12-binding proteins were almost exclusively localized to the specific granules. Chromatographic analysis of these proteins showed them to have approximately equal proportions of transcobalamins I and III. Vitamin B12 assayed microbiologically could not be detected in the specific granule fractions.<\/jats:p>","DOI":"10.1042\/cs0490171","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T10:29:47Z","timestamp":1438943387000},"page":"171-182","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analytical Subcellular Fractionation of Human Granulocytes with Reference to the Localization of Vitamin B12-Binding Proteins"],"prefix":"10.1042","volume":"49","author":[{"given":"S. P.","family":"Kane","sequence":"first","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}]},{"given":"T. J.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Medicine, Royal Postgraduate Medical School, London"}]}],"member":"288","container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/49\/2\/171\/815629\/cs0490171.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/portlandpress.com\/clinsci\/article-pdf\/49\/2\/171\/815629\/cs0490171.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T21:03:36Z","timestamp":1571951016000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/49\/2\/171\/70463\/Analytical-Subcellular-Fractionation-of-Human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,8,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/cs0490171","relation":{},"ISSN":["0301-0538"],"issn-type":[{"value":"0301-0538","type":"print"}],"subject":[],"published":{"date-parts":[[1975,8,1]]}}}