{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:22:30Z","timestamp":1760887350271},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":2701,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p>Four inducible promoters, <jats:italic>uspA<\/jats:italic>, <jats:italic>uspB<\/jats:italic>, <jats:italic>lacUV5<\/jats:italic> and <jats:italic>malK<\/jats:italic>, were evaluated in the expression of the fusion protein ZZ\u2010proinsulin by <jats:italic>Escherichia coli<\/jats:italic>. The aim was to select for their effects on the most appropriate expression system (promoter and culture medium) for secretion of ZZ\u2010proinsulin to the periplasmic space and culture medium. All the expression vectors contained the RNase III cleavage site to ensure that the mRNA translation rate remained independent of 5\u2032\u2010untranslated regions thus making promoter strength comparisons more accurate. The highest ZZ\u2010proinsulin secretion yields were 6.2 mg\/g of dry cell weight in the periplasmic space and 2.6 mg\/g of dry cell weight in the culture medium using the <jats:italic>malK<\/jats:italic> promoter. It was also demonstrated that the use of M9 minimal medium favours secretion.<\/jats:p>","DOI":"10.1042\/ba20030043","type":"journal-article","created":{"date-parts":[[2003,9,10]],"date-time":"2003-09-10T13:13:55Z","timestamp":1063199635000},"page":"87-93","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of inducible promoters on the secretion of a ZZ\u2010proinsulin fusion protein in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"38","author":[{"given":"Filipe J. M.","family":"Mergulh\u00e3o","sequence":"first","affiliation":[]},{"given":"Gabriel A.","family":"Monteiro","sequence":"additional","affiliation":[]},{"given":"Gen","family":"Larsson","sequence":"additional","affiliation":[]},{"given":"Maria","family":"Bostrom","sequence":"additional","affiliation":[]},{"given":"Anne","family":"Farewell","sequence":"additional","affiliation":[]},{"given":"Thomas","family":"Nystr\u00f6m","sequence":"additional","affiliation":[]},{"given":"Joaquim M. 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