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A chimaeric protein was constructed by fusion of SIV matrix protein (p17) and HIV1 p6 protein, and we demonstrated that the chimaeric proteins assemble as 80 nm nanoparticles containing \u223c7700 chimaeric protein units. Chimaeric VLPs are released from HEK\u2010293T cells (human embryonic kidney cells expressing the large T\u2010antigen of simian virus 40) and are fully encapsulated with lipid membrane. Chimaeric VLPs are produced at 3.7\u2010fold higher levels when compared with SIV p17 VLPs owing to duplication of a PTAP (Pro\u2010Thr\u2010Ala\u2010Pro) domain previously shown as essential for virus particle release. The chimaeric VLPs constructed in the present paper were efficiently pseudotyped with vesicular\u2010stomatitis\u2010virus glycoprotein, as shown by immunoprecipitation assays.<\/jats:p>","DOI":"10.1042\/ba20060208","type":"journal-article","created":{"date-parts":[[2007,8,28]],"date-time":"2007-08-28T15:38:00Z","timestamp":1188315480000},"page":"35-43","source":"Crossref","is-referenced-by-count":4,"title":["Molecular construction of bionanoparticles: chimaeric SIV p17\u2013HIV I p6 nanoparticles with minimal viral protein content"],"prefix":"10.1002","volume":"48","author":[{"given":"Maria J. L.","family":"Costa","sequence":"first","affiliation":[]},{"given":"Lu\u00edsa","family":"Pedro","sequence":"additional","affiliation":[]},{"given":"Ant\u00f3nio P. 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