{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T05:27:10Z","timestamp":1759814830153},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,12,23]],"date-time":"2010-12-23T00:00:00Z","timestamp":1293062400000},"content-version":"vor","delay-in-days":509,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotech and App Biochem"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:p>Micrometre\u2010sized aggregates of a 6050\u2010bp plasmid obtained by the addition of 1.5\u20133.0 mM CaCl<jats:sub>2<\/jats:sub> and 20% (v\/v) t\u2010butanol or 0.3\u20131.0% (v\/v) APG (aluminium phosphate gel) were subjected to degradation induced by sonication or vortex flows. Dynamic light scattering revealed that the plasmid hydrodynamic radius increases from 116 nm to &gt;1300 nm and approx. 1000 nm, when formulated with CaCl<jats:sub>2<\/jats:sub>\/t\u2010butanol and APG respectively. CD showed that addition of CaCl<jats:sub>2<\/jats:sub>\/t\u2010butanol leads to transition in plasmid structure from B\u2010DNA to a \u03c8\u2010DNA negative form, whereas no detectable transitions were observed for APG formulations. The ability of the condensing agents to stabilize supercoiled plasmid isoforms subjected to sonication or turbulent Taylor vortices was assessed by agarose\u2010gel electrophoresis. Although naked plasmid was completely fragmented after 5 s of sonication, condensing agents increased the plasmid stability dramatically [e.g. up to 80% after 30 s with 1.5 mM CaCl<jats:sub>2<\/jats:sub>+20% (v\/v) t\u2010butanol]. In the case of the vorticular flow system, the extent of degradation correlated well with the shear stress associated with flow of the solutions being processed. Overall, the results from the present study demonstrate that condensing agents such as CaCl<jats:sub>2<\/jats:sub>\/t\u2010butanol and APG can effectively stabilize plasmids against shear\u2010induced degradation; the extent of protection, however, depends on both the condensing agents and the shear\u2010inducing system used.<\/jats:p>","DOI":"10.1042\/ba20080215","type":"journal-article","created":{"date-parts":[[2009,2,20]],"date-time":"2009-02-20T13:51:47Z","timestamp":1235137907000},"page":"237-246","source":"Crossref","is-referenced-by-count":9,"title":["Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high\u2010shear vortices"],"prefix":"10.1002","volume":"53","author":[{"given":"Ming L.","family":"Wu","sequence":"first","affiliation":[]},{"given":"Sind\u00e9lia S.","family":"Freitas","sequence":"additional","affiliation":[]},{"given":"Gabriel A.","family":"Monteiro","sequence":"additional","affiliation":[]},{"given":"Duarte M. F.","family":"Prazeres","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9 A. L.","family":"Santos","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2010,12,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.10140"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004490050552"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp070066z"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaerosci.2004.11.017"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470117118.ch4a"},{"key":"e_1_2_7_7_2","first-page":"1560","volume":"45","author":"Davinson P. F.","year":"1959","journal-title":"Biochemistry"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/jam.2006.19.372"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(00)01446-3"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(92)81831-3"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0927-7765(99)00005-3"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360311305"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg936"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3470"},{"key":"e_1_2_7_15_2","doi-asserted-by":"crossref","first-page":"103","DOI":"10.18388\/abp.2000_4067","volume":"47","author":"Hackl E. V.","year":"2000","journal-title":"Acta Biochim. Pol."},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1093"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.8.1886"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2005.03.004"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(99)00041-1"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.memsci.2006.03.003"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp050280s"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(03)00367-3"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/js9504752"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/js990042p"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-5173(03)00107-8"},{"key":"e_1_2_7_26_2","first-page":"3","volume":"9","author":"Chaszewska\u2010Makowska M.","year":"2004","journal-title":"Cell. Mol. Biol. Lett."},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1350-4177(03)00101-9"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008814551841"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302642"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012198321879"},{"key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1128\/aem.57.8.2197-2204.1991","volume":"57","author":"Paul J. H.","year":"1991","journal-title":"Environ. Microbiol."},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.218"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0376-7388(93)85232-L"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.1989.tb02831.x"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(98)00008-8"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(95)00011-O"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00578a009"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp972780c"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1088\/0953-8984\/12\/8A\/313"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.13.3228"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/bip.360211105"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki140"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.11.6808"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.13.2795"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01053-4"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-003-0484-1"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.5.1297"},{"key":"e_1_2_7_48_2","first-page":"4821","volume":"23","author":"Porscheke D.","year":"1984","journal-title":"Biopolymers"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1517\/17425247.1.1.37"},{"key":"e_1_2_7_50_2","doi-asserted-by":"publisher","DOI":"10.1289\/ehp.8564233"},{"key":"e_1_2_7_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(62)86852-0"},{"key":"e_1_2_7_52_2","doi-asserted-by":"publisher","DOI":"10.1134\/S0026893306020142"},{"key":"e_1_2_7_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/jps.20511"},{"key":"e_1_2_7_54_2","doi-asserted-by":"publisher","DOI":"10.1021\/js9800477"},{"key":"e_1_2_7_55_2","first-page":"168","volume":"67","author":"Ma C.","year":"1994","journal-title":"Biophys. J."},{"key":"e_1_2_7_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.23.5295"}],"container-title":["Biotechnology and Applied Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1042%2FBA20080215","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/pdf\/10.1042\/BA20080215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T23:19:18Z","timestamp":1697411958000},"score":1,"resource":{"primary":{"URL":"https:\/\/iubmb.onlinelibrary.wiley.com\/doi\/10.1042\/BA20080215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,8]]},"references-count":55,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,8]]}},"alternative-id":["10.1042\/BA20080215"],"URL":"https:\/\/doi.org\/10.1042\/ba20080215","archive":["Portico"],"relation":{},"ISSN":["0885-4513","1470-8744"],"issn-type":[{"value":"0885-4513","type":"print"},{"value":"1470-8744","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,8]]}}}