{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T03:54:46Z","timestamp":1767585286713},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,1,9]],"date-time":"2012-01-09T00:00:00Z","timestamp":1326067200000},"content-version":"vor","delay-in-days":2413,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology of the Cell"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p><jats:italic>Background information<\/jats:italic>. NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro\u2010angiogenic effects and potentiation of noradrenaline\u2010induced vasoconstriction. In HUVEC (human umbilical\u2010vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis, storage and secretion have not been studied. The aim of the present study was to establish NPY expression, storage and cellular transducing effects in HUVEC.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>. HUVEC contain 0.19 fmol of NPY\/\u03bcg of protein and 0.46 fmol of pro\u2010NPY\/\u03bcg of protein, as measured by ELISA. RT (reverse transcriptase)\u2014PCR confirmed the expression of NPY in HUVEC. Immunofluorescence revealed the presence of NPY in small punctate structures, with a fluorescence pattern different from that observed for von Willebrand factor, indicating distinct storage compartments. Double labelling for NPY and Rab3A demonstrated similar granular patterns, with at least partial co\u2010localization. Electron microscopy showed NPY immunoreactivity in vesicle\u2010like cytoplasmic structures, of a fine fibrillar texture, as well as in mitochondria and in the nucleus. A similar general distribution pattern was also obtained for Rab3A. Y1 and Y2 receptors were expressed in HUVEC as assessed by RT\u2014PCR, and they were functional since NPY induced a 42 nM intracellular calcium increase within 100 s, representing 22% of the histamine\u2010induced response. In contrast with histamine, NPY did not induce acute von Willebrand factor secretion.<\/jats:p><jats:p><jats:italic>Conclusions<\/jats:italic>. HUVEC produce, store and respond to NPY, suggesting an autocrine regulatory role for NPY in the endothelium.<\/jats:p>","DOI":"10.1042\/bc20040102","type":"journal-article","created":{"date-parts":[[2008,5,8]],"date-time":"2008-05-08T10:33:15Z","timestamp":1210242795000},"page":"457-467","source":"Crossref","is-referenced-by-count":23,"title":["Neuropeptide Y expression, localization and cellular transducing effects in HUVEC"],"prefix":"10.1111","volume":"97","author":[{"given":"Antonio P.","family":"Silva","sequence":"first","affiliation":[]},{"given":"Jocelyne E.","family":"Kaufmann","sequence":"additional","affiliation":[]},{"given":"C\u00e9cile","family":"Vivancos","sequence":"additional","affiliation":[]},{"given":"Stanislav","family":"Fakan","sequence":"additional","affiliation":[]},{"given":"Claudia","family":"Cavadas","sequence":"additional","affiliation":[]},{"given":"Phillip","family":"Shaw","sequence":"additional","affiliation":[]},{"given":"Hans R.","family":"Brunner","sequence":"additional","affiliation":[]},{"given":"Ulrich","family":"Vischer","sequence":"additional","affiliation":[]},{"given":"Eric","family":"Grouzmann","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2012,1,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/306584a0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V73.5.1109.1109"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00919.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00323584"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00314550"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1210\/jcem.86.12.8091"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00225"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)00219-4"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(92)90038-G"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(94)90006-X"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701808"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(94)90180-5"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00242-8"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(01)00340-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1992.11.409"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(92)90004-M"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.12.7699"},{"key":"e_1_2_7_19_1","first-page":"27033","article-title":"Erratum","volume":"273","author":"Grouzmann E.","year":"1998","journal-title":"J. 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