{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T08:38:19Z","timestamp":1773477499890,"version":"3.50.1"},"reference-count":36,"publisher":"Portland Press Ltd.","issue":"21","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>CoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility\u2013mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.<\/jats:p>","DOI":"10.1042\/bcj20190318","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T15:45:17Z","timestamp":1567698317000},"page":"3125-3139","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Structural insights into <i>Escherichia coli<\/i> phosphopantothenoylcysteine synthetase by native ion mobility\u2013mass spectrometry"],"prefix":"10.1042","volume":"476","author":[{"given":"Daniel Shiu-Hin","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5916-0728","authenticated-orcid":false,"given":"Jeannine","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"given":"Elen","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-7070","authenticated-orcid":false,"given":"Christina","family":"Spry","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"given":"Robert","family":"Starley","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"given":"Claudio","family":"Dagostin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K."}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5312-0191","authenticated-orcid":false,"given":"Marcio V. 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