{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T00:57:45Z","timestamp":1778288265949,"version":"3.51.4"},"reference-count":23,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2006,5,1]]},"abstract":"<jats:p>Candida intermedia PYCC 4715 was previously shown to grow well on xylose and to transport this sugar by two different transport systems: high-capacity and low-affinity facilitated diffusion and a high-affinity xylose\u2013proton symporter, both of which accept glucose as a substrate. Here we report the isolation of genes encoding both transporters, designated GXF1 (glucose\/xylose facilitator 1) and GXS1 (glucose\/xylose symporter 1) respectively. Although GXF1 was isolated by functional complementation of an HXT-null (where Hxt refers to hexose transporters) Saccharomyces cerevisiae strain, isolation of the GXS1 cDNA required partial purification and micro-sequencing of the transporter, identified by its relative abundance in cells grown on low xylose concentrations. Both genes were expressed in S. cerevisiae and the kinetic parameters of glucose and xylose transport were determined. Gxs1 is the first yeast xylose\/glucose\u2013H+ symporter to be characterized at the molecular level. Comparison of its amino acid sequence with available sequence data revealed the existence of a family of putative monosaccharide\u2013H+ symporters encompassing proteins from several yeasts and filamentous fungi.<\/jats:p>","DOI":"10.1042\/bj20051465","type":"journal-article","created":{"date-parts":[[2006,4,11]],"date-time":"2006-04-11T16:45:03Z","timestamp":1144773903000},"page":"543-549","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":142,"title":["Two glucose\/xylose transporter genes from the yeast <i>Candida intermedia<\/i>: first molecular characterization of a yeast xylose\u2013H+ symporter"],"prefix":"10.1042","volume":"395","author":[{"given":"Maria\u00a0Jos\u00e9","family":"Leandro","sequence":"first","affiliation":[{"name":"Centro de Recursos Microbiol\u00f3gicos (CREM), Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Caparica, Portugal"}]},{"given":"Paula","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Centro de Recursos Microbiol\u00f3gicos (CREM), Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Caparica, Portugal"}]},{"given":"Isabel","family":"Spencer-Martins","sequence":"additional","affiliation":[{"name":"Centro de Recursos Microbiol\u00f3gicos (CREM), Biotechnology Unit, Faculty of Sciences and Technology, New University of Lisbon, 2829-516 Caparica, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2006,4,11]]},"reference":[{"key":"2021112214122047000_B1","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1007\/s00253-003-1444-y","article-title":"Bacteria engineered for fuel ethanol production: current status","volume":"63","author":"Dien","year":"2003","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2021112214122047000_B2","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s00253-003-1450-0","article-title":"Metabolic engineering for improved fermentation of pentoses by yeasts","volume":"70","author":"Jeffries","year":"2004","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"2021112214122047000_B3","first-page":"231","article-title":"Pentose fermentation to alcohol","volume-title":"Bioconversion of Forest and Agricultural Plant Residues","author":"Hahn-H\u00e4gerdal","year":"1993"},{"key":"2021112214122047000_B4","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/0005-2736(67)90013-2","article-title":"Mobility of the free and of the loaded monosaccharide carrier in Saccharomyces cerevisiae","volume":"135","author":"Kotyk","year":"1967","journal-title":"Biochim. Biophys. Acta"},{"key":"2021112214122047000_B5","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1002\/bit.10631","article-title":"Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae","volume":"82","author":"G\u00e1rdonyi","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"2021112214122047000_B6","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/yea.1060","article-title":"Characterization of the effectiveness of hexose transporters for transporting xylose during glucose and xylose co-fermentation by a recombinant Saccharomyces yeast","volume":"21","author":"Sedlak","year":"2004","journal-title":"Yeast"},{"key":"2021112214122047000_B7","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1046\/j.1365-2958.1999.01224.x","article-title":"Cloning and characterization of three genes (SUT1\u2013SUT3) encoding glucose transporters of the yeast Pichia stipitis","volume":"31","author":"Weierstall","year":"1999","journal-title":"Mol. Microbiol."},{"key":"2021112214122047000_B8","first-page":"45","article-title":"High capacity xylose transport in Candida intermedia PYCC 4715","volume":"3","author":"G\u00e1rdonyi","year":"2003","journal-title":"FEMS Yeast Res."},{"key":"2021112214122047000_B9","doi-asserted-by":"crossref","first-page":"2783","DOI":"10.1099\/00221287-148-9-2783","article-title":"Characterization of the xylose-transporting properties of yeast hexose transporters and their influence on xylose utilization","volume":"148","author":"Hamacher","year":"2002","journal-title":"Microbiology"},{"key":"2021112214122047000_B10","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/0378-1119(88)90185-0","article-title":"New yeast\u2013Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites","volume":"74","author":"Gietz","year":"1988","journal-title":"Gene"},{"key":"2021112214122047000_B11","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/S0076-6879(02)50957-5","article-title":"Transformation of yeast by lithium acetate\/single-stranded carrier DNA\/polyethylene glycol method","volume":"350","author":"Gietz","year":"2002","journal-title":"Methods Enzymol."},{"key":"2021112214122047000_B12","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/0005-2736(85)90535-8","article-title":"Catabolite interconversion of glucose transport systems in the yeast Candida wickerhamii","volume":"812","author":"Spencer-Martins","year":"1985","journal-title":"Biochim. Biophys. Acta"},{"key":"2021112214122047000_B13","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/yea.320080703","article-title":"Effect of benzoic acid on metabolic fluxes in yeasts: a continuous culture study on the regulation of respiration and alcoholic fermentation","volume":"8","author":"Verduyn","year":"1992","journal-title":"Yeast"},{"key":"2021112214122047000_B14","doi-asserted-by":"crossref","first-page":"12146","DOI":"10.1016\/S0021-9258(18)98871-X","article-title":"Proton-motive force-driven D-galactose transport in plasma membrane vesicles from the yeast Kluyveromyces marxianus","volume":"266","author":"Van\u00a0Leeuwen","year":"1991","journal-title":"J. Biol. Chem."},{"key":"2021112214122047000_B15","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"2021112214122047000_B16","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/B978-0-08-057172-0.50010-3","article-title":"Denaturing electrophoretic techniques","volume-title":"A Practical Guide to Membrane Protein Purification","author":"Sch\u00e4gger","year":"1994"},{"key":"2021112214122047000_B17","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1099\/13500872-142-8-2279","article-title":"Ribosomal protein gene transcription in Saccharomyces cerevisiae shows a biphasic response to nutritional changes","volume":"142","author":"Griffioen","year":"1996","journal-title":"Microbiology"},{"key":"2021112214122047000_B18","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/S0014-5793(98)01583-X","article-title":"Catabolite inactivation of the high-affinity hexose transporters Hxt6 and Hxt7 of Saccharomyces cerevisiae occurs in the vacuole after internalization by endocytosis","volume":"441","author":"Krampe","year":"1998","journal-title":"FEBS Lett."},{"key":"2021112214122047000_B19","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/0076-6879(90)85029-N","article-title":"High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene","volume":"185","author":"Kingsman","year":"1990","journal-title":"Methods Enzymol."},{"key":"2021112214122047000_B20","article-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989","edition":"2nd edn"},{"key":"2021112214122047000_B21","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein database search programs","volume":"25","author":"Altschul","year":"1997","journal-title":"Nucleic Acids Res."},{"key":"2021112214122047000_B22","doi-asserted-by":"crossref","first-page":"15264","DOI":"10.1016\/S0021-9258(17)42544-0","article-title":"L(+)-Lactate binding to preparations of rat hepatocyte plasma membranes","volume":"259","author":"Welch","year":"1984","journal-title":"J. Biol. Chem."},{"key":"2021112214122047000_B23","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1042\/bj20030624","article-title":"Aspergillus niger mstA encodes a high-affinity sugar\/H+ symporter which is regulated in response to extracellular pH","volume":"379","author":"Vankuyk","year":"2004","journal-title":"Biochem. J."}],"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/395\/3\/543\/642517\/bj3950543.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/395\/3\/543\/642517\/bj3950543.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T16:51:13Z","timestamp":1637599873000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/395\/3\/543\/44179\/Two-glucose-xylose-transporter-genes-from-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,11]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20051465","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,11]]}}}