{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:38:39Z","timestamp":1777448319366,"version":"3.51.4"},"reference-count":50,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2007,2,15]]},"abstract":"<jats:p>PP1 (protein phosphatase 1) is a ubiquitously expressed serine\/threonine-specific protein phosphatase whose activity towards different substrates appears to be mediated via binding to specific proteins that play critical regulatory and targeting roles. In the present paper we report the cloning and characterization of a new protein, termed SARP (several ankyrin repeat protein), which is shown to interact with all isoforms of PP1 by a variety of techniques. A region encompassing a consensus PP1-binding motif in SARP (K354VHF357) modulates endogenous SARP\u2013PP1 activity in mammalian cells. This SARP\u2013PP1 interaction motif lies partially within the first ankyrin repeat in contrast with other proteins [53BP2 (p53 binding protein 2), MYPT1\/M110\/MBS (myosin binding protein of PP1) and TIMAP (transforming growth factor \u03b2 inhibited, membrane-associated protein)], where a PP1-binding motif precedes the ankyrin repeats. Alternative mRNA splicing produces several isoforms of SARP from a single human gene at locus 11q14. SARP1 and\/or SARP2 (92\u201395\u00a0kDa) are ubiquitously expressed in all tissues with high levels in testis and sperm, where they are shown to interact with both PP1\u03b31 and PP1\u03b32. SARP3 (65\u00a0kDa) is most abundant in brain where SARP isoforms interact with both PP1\u03b1 and PP1\u03b31. SARP is highly abundant in the nucleus of mammalian cells, consistent with the putative nuclear localization signal at the N-terminus. The presence of a leucine zipper near the C-terminus of SARP1 and SARP2, and the binding of mammalian DNA to SARP2, suggests that SARP1 and SARP2 may be transcription factors or DNA-associated proteins that modulate gene expression.<\/jats:p>","DOI":"10.1042\/bj20060600","type":"journal-article","created":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T14:42:48Z","timestamp":1169736168000},"page":"187-196","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["SARP, a new alternatively spliced protein phosphatase 1 and DNA interacting protein"],"prefix":"10.1042","volume":"402","author":[{"given":"Gareth\u00a0J.","family":"Browne","sequence":"first","affiliation":[{"name":"Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}]},{"given":"Margarida","family":"Fardilha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Senga\u00a0K.","family":"Oxenham","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}]},{"given":"Wenjuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Nicholas\u00a0R.","family":"Helps","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}]},{"given":"Odete\u00a0A.\u00a0B.","family":"da\u00a0Cruz\u00a0E\u00a0Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroci\u00eancias, Centro de Biologia Celular, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]},{"given":"Patricia\u00a0T.\u00a0W.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Medical Research Council Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, U.K."}]},{"given":"Edgar\u00a0F.\u00a0da","family":"Cruz\u00a0E\u00a0Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Transdu\u00e7\u00e3o de Sinais, Universidade de Aveiro, 3810-193 Aveiro, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2007,1,25]]},"reference":[{"key":"2021112213060228900_B1","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1242\/jcs.115.2.241","article-title":"Protein phosphatase 1-targeted in many directions","volume":"115","author":"Cohen","year":"2002","journal-title":"J. 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