{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:31:23Z","timestamp":1773657083333,"version":"3.50.1"},"reference-count":52,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,12,15]]},"abstract":"<jats:p>Protein glycation is involved in structure and stability changes that impair protein functionality, which is associated with several human diseases, such as diabetes and amyloidotic neuropathies (Alzheimer's disease, Parkinson's disease and Andrade's syndrome). To understand the relationship of protein glycation with protein dysfunction, unfolding and \u03b2-fibre formation, numerous studies have been carried out in vitro. All of these previous experiments were conducted in non-physiological or pseudo-physiological conditions that bear little to no resemblance to what may happen in a living cell. In vivo, glycation occurs in a crowded and organized environment, where proteins are exposed to a steady-state of glycation agents, namely methylglyoxal, whereas in vitro, a bolus of a suitable glycation agent is added to diluted protein samples. In the present study, yeast was shown to be an ideal model to investigate glycation in vivo since it shows different glycation phenotypes and presents specific protein glycation targets. A comparison between in vivo glycated enolase and purified enolase glycated in vitro revealed marked differences. All effects regarding structure and stability changes were enhanced when the protein was glycated in vitro. The same applies to enzyme activity loss, dimer dissociation and unfolding. However, the major difference lies in the nature and location of specific advanced glycation end-products. In vivo, glycation appears to be a specific process, where the same residues are consistently modified in the same way, whereas in vitro several residues are modified with different advanced glycation end-products.<\/jats:p>","DOI":"10.1042\/bj20080632","type":"journal-article","created":{"date-parts":[[2008,7,25]],"date-time":"2008-07-25T14:14:40Z","timestamp":1216995280000},"page":"317-326","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Protein glycation<i>in vivo<\/i>: functional and structural effects on yeast enolase"],"prefix":"10.1042","volume":"416","author":[{"given":"Ricardo\u00a0A.","family":"Gomes","sequence":"first","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]},{"given":"Lu\u00eds\u00a0M.\u00a0A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"},{"name":"Laborat\u00f3rio de Patologias Neurodegenerativas, Instituto Superior de Ci\u00eancias da Sa\u00fade Egas Moniz, 2829-511 Monte da Caparica, Portugal"}]},{"given":"Mariana","family":"Silva","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]},{"given":"Carla","family":"Ascenso","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologias Neurodegenerativas, Instituto Superior de Ci\u00eancias da Sa\u00fade Egas Moniz, 2829-511 Monte da Caparica, Portugal"}]},{"given":"Alexandre","family":"Quintas","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Patologias Neurodegenerativas, Instituto Superior de Ci\u00eancias da Sa\u00fade Egas Moniz, 2829-511 Monte da Caparica, Portugal"}]},{"given":"Gon\u00e7alo","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Espectrometria de Massa do Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"}]},{"given":"Ana\u00a0V.","family":"Coelho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Espectrometria de Massa do Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal"},{"name":"Departamento de Qu\u00edmica da Universidade de \u00c9vora, 7004-516 \u00c9vora, Portugal"}]},{"given":"Marta","family":"Sousa\u00a0Silva","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]},{"given":"Ant\u00f3nio\u00a0E.\u00a0N.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]},{"given":"Ana","family":"Ponces\u00a0Freire","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]},{"given":"Carlos","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"Centro de Qu\u00edmica e Bioqu\u00edmica, Departamento de Qu\u00edmica e Bioqu\u00edmica, Faculdade de Ci\u00eancias da Universidade de Lisboa, Edif\u00edcio C8, 1749-016 Lisboa, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2008,11,26]]},"reference":[{"key":"2021112215084311300_B1","first-page":"57","article-title":"Glycation and advanced glycation endproducts","volume-title":"The Glycation Hypothesis of Atherosclerosis","author":"Westwood","year":"1997"},{"key":"2021112215084311300_B2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s00726-007-0606-0","article-title":"Advanced glycation end-products (AGEs): involvement in aging and in neurodegenerative diseases","volume":"35","author":"Grillo","year":"2008","journal-title":"Amino Acids"},{"key":"2021112215084311300_B3","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1146\/annurev.med.46.1.223","article-title":"Advanced protein glycosylation in diabetes and aging","volume":"46","author":"Brownlee","year":"1995","journal-title":"Annu. 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