{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:52:46Z","timestamp":1762113166257},"reference-count":29,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,6,15]]},"abstract":"<jats:p>Multihaem cytochromes that could form protein \u201cnanowires\u201d were identified in the Geobacter sulfurreducens genome, and represent a new type of multihaem cytochrome. The sequences of these proteins, two with 12 haems (GSU1996, GSU0592) and one with 27 haems (GSU2210), suggest that they are formed with domains homologous to the trihaem cytochrome c7. Although all three haems have bis-His co-ordination in cytochromes c7, in each domain of the above polymers, the haem equivalent to haem IV has His-Met co-ordination. We previously determined the structure and measured the macroscopic redox potential of one representative domain (domain C) of a dodecahaem cytochrome (GSU1996). In the present study, the microscopic redox properties of the individual haem groups of domain C were determined using NMR and UV\u2013visible spectroscopies. The reduction potentials of the haems for the fully reduced and protonated protein are different from each other (haem I, \u2212106\u00a0mV; haem III, \u2212136\u00a0mV; and haem IV, \u2212125\u00a0mV) and are strongly modulated by redox interactions. This result is rather surprising since the His-Met co-ordinated haem IV does not have the highest potential as was expected. The polypeptide environment of each haem group and the strong haem pairwise redox interactions must play a dominant role in controlling the individual haem potentials. The strong redox interactions between the haems extend the range of their operating potentials at physiological pH (haem I, \u221271\u00a0mV, haem III, \u2212146\u00a0mV and haem IV, \u2212110\u00a0mV). Such a modulation in haem potentials is likely to have a functional significance in the metabolism of G. sulfurreducens.<\/jats:p>","DOI":"10.1042\/bj20082428","type":"journal-article","created":{"date-parts":[[2009,4,8]],"date-time":"2009-04-08T09:38:55Z","timestamp":1239183535000},"page":"485-492","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Thermodynamic characterization of the redox centres in a representative domain of a novel <i>c<\/i>-type multihaem cytochrome"],"prefix":"10.1042","volume":"420","author":[{"given":"Leonor","family":"Morgado","sequence":"first","affiliation":[{"name":"Requimte-CQFB, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Ana\u00a0P.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Requimte-CQFB, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal"}]},{"given":"Yuri\u00a0Y.","family":"Londer","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, U.S.A."}]},{"given":"P.\u00a0Raj","family":"Pokkuluri","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, U.S.A."}]},{"given":"Marianne","family":"Schiffer","sequence":"additional","affiliation":[{"name":"Biosciences Division, Argonne National Laboratory, Argonne, IL 60439, U.S.A."}]},{"given":"Carlos\u00a0A.","family":"Salgueiro","sequence":"additional","affiliation":[{"name":"Requimte-CQFB, Departamento de Qu\u00edmica, Faculdade de Ci\u00eancias e Tecnologia, Universidade Nova de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2009,5,27]]},"reference":[{"key":"2021112215103173200_B1","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/S0958-1669(00)00207-X","article-title":"Microbial detoxification of metals and radionuclides","volume":"12","author":"Lloyd","year":"2001","journal-title":"Curr. 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