{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:11:13Z","timestamp":1776075073018,"version":"3.50.1"},"reference-count":22,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:p>On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a \u2018saturable\u2019 component of the transport, which requires the presence of a functional Jen1p transporter, and a \u2018non-saturable\u2019 component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.<\/jats:p>","DOI":"10.1042\/bj20120312","type":"journal-article","created":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T08:29:14Z","timestamp":1372062554000},"page":"551-558","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies"],"prefix":"10.1042","volume":"454","author":[{"given":"Sandra","family":"Paiva","sequence":"first","affiliation":[{"name":"Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Dita","family":"Strachotov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the AS CR, v.v.i., V\u00edde\u0148sk\u00e1 1083, 142 20 Prague 4, Czech Republic"},{"name":"Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vini\u010dn\u00e1 5, 128 44 Prague 2, Czech Republic"}]},{"given":"Helena","family":"Ku\u010derov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the AS CR, v.v.i., V\u00edde\u0148sk\u00e1 1083, 142 20 Prague 4, Czech Republic"},{"name":"Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vini\u010dn\u00e1 5, 128 44 Prague 2, Czech Republic"}]},{"given":"Otakar","family":"Hlav\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the AS CR, v.v.i., V\u00edde\u0148sk\u00e1 1083, 142 20 Prague 4, Czech Republic"}]},{"given":"Sandra","family":"Mota","sequence":"additional","affiliation":[{"name":"Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal"},{"name":"Centre of Health and Environmental Research CISA, Superior School of Health Technology of Porto, Polytechnic Institute of Porto, 4200 Porto, Portugal"}]},{"given":"Margarida","family":"Casal","sequence":"additional","affiliation":[{"name":"Molecular and Environmental Biology Centre (CBMA), Department of Biology, Campus de Gualtar, University of Minho, 4710-057 Braga, Portugal"}]},{"given":"Zdena","family":"Palkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Vini\u010dn\u00e1 5, 128 44 Prague 2, Czech Republic"}]},{"given":"Libu\u0161e","family":"V\u00e1chov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the AS CR, v.v.i., V\u00edde\u0148sk\u00e1 1083, 142 20 Prague 4, Czech Republic"}]}],"member":"288","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"2021112215440679200_B1","doi-asserted-by":"crossref","first-page":"3901","DOI":"10.1091\/mbc.e01-12-0149","article-title":"Ammonia pulses and metabolic oscillations guide yeast colony development","volume":"13","author":"Palkova","year":"2002","journal-title":"Mol. 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