{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T10:09:34Z","timestamp":1773396574864,"version":"3.50.1"},"reference-count":52,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:p>Laforin is a human dual-specificity phosphatase (DSP) involved in glycogen metabolism regulation containing a carbohydrate-binding module (CBM). Mutations in the gene coding for laforin are responsible for the development of Lafora disease, a progressive fatal myoclonus epilepsy with early onset, characterized by the intracellular deposition of abnormally branched, hyperphosphorylated insoluble glycogen-like polymers, called Lafora bodies. Despite the known importance of the CBM domain of laforin in the regulation of glycogen metabolism, the molecular mechanism of laforin\u2013glycogen interaction is still poorly understood. Recently, the structure of laforin with bound maltohexaose was determined and despite the importance of such breakthrough, some molecular interaction details remained missing. We herein report a thorough biophysical characterization of laforin\u2013carbohydrate interaction using soluble glycans. We demonstrated an increased preference of laforin for the interaction with glycans with higher order of polymerization and confirmed the importance of tryptophan residues for glycan interaction. Moreover, and in line with what has been described for other CBMs and lectins, our results confirmed that laforin\u2013glycan interactions occur with a favourable enthalpic contribution counter-balanced by an unfavourable entropic contribution. The analysis of laforin\u2013glycan interaction through the glycan side by saturation transfer difference (STD)\u2013NMR has shown that the CBM-binding site can accommodate between 5 and 6 sugar units, which is in line with the recently obtained crystal structure of laforin. Overall, the work in the present study complements the structural characterization of laforin and sheds light on the molecular mechanism of laforin\u2013glycan interaction, which is a pivotal requisite to understand the physiological and pathological roles of laforin.<\/jats:p>","DOI":"10.1042\/bj20141555","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T02:11:25Z","timestamp":1447812685000},"page":"335-345","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Biophysical characterization of laforin\u2013carbohydrate interaction"],"prefix":"10.1042","volume":"473","author":[{"given":"David\u00a0M.","family":"Dias","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, U.K."},{"name":"Coimbra Chemistry Center, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"Department of Life Sciences, Faculty of Science and Technology, University of Coimbra, 3000-393 Coimbra, Portugal"}]},{"given":"Joana","family":"Furtado","sequence":"additional","affiliation":[{"name":"Biocant\u2013Biotechnology Innovation Center, Parque Tecnol\u00f3gico de Cantanhede, N\u00facleo 4, Lote 8\u20133060-197 Cantanhede, Portugal"}]},{"given":"Emeric","family":"Wasielewski","sequence":"additional","affiliation":[{"name":"Coimbra Chemistry Center, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"given":"Rui","family":"Cruz","sequence":"additional","affiliation":[{"name":"Biocant\u2013Biotechnology Innovation Center, Parque Tecnol\u00f3gico de Cantanhede, N\u00facleo 4, Lote 8\u20133060-197 Cantanhede, Portugal"}]},{"given":"Bernard","family":"Costello","sequence":"additional","affiliation":[{"name":"Applied Photophysics Ltd., 21 Mole Business Park, Leatherhead, Surrey KT22 7BA, U.K."}]},{"given":"Lindsay","family":"Cole","sequence":"additional","affiliation":[{"name":"Applied Photophysics Ltd., 21 Mole Business Park, Leatherhead, Surrey KT22 7BA, U.K."}]},{"given":"Tiago\u00a0Q.","family":"Faria","sequence":"additional","affiliation":[{"name":"CNC\u2013Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Philipp","family":"Baaske","sequence":"additional","affiliation":[{"name":"Nanotemper Technologies GmbH, Fl\u00f6\u00dfergasse 4, 81369 Munich, Germany"}]},{"given":"Rui\u00a0M. \u00a0M.","family":"Brito","sequence":"additional","affiliation":[{"name":"Coimbra Chemistry Center, University of Coimbra, 3004-535 Coimbra, Portugal"},{"name":"CNC\u2013Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, 3004-517 Coimbra, Portugal"},{"name":"Chemistry Department, Faculty of Science and Technology, University of Coimbra, 3004-535 Coimbra, Portugal"}]},{"given":"Alessio","family":"Ciulli","sequence":"additional","affiliation":[{"name":"College of Life Sciences, University of Dundee, Division of Biological Chemistry and Drug Discovery, James Black Centre, Dow Street, Dundee, DD1 5EH, U.K."}]},{"given":"Isaura","family":"Sim\u00f5es","sequence":"additional","affiliation":[{"name":"Biocant\u2013Biotechnology Innovation Center, Parque Tecnol\u00f3gico de Cantanhede, N\u00facleo 4, Lote 8\u20133060-197 Cantanhede, Portugal"},{"name":"CNC\u2013Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Sandra","family":"Macedo-Ribeiro","sequence":"additional","affiliation":[{"name":"IBMC\u2013Institute for Molecular and Cell Biology, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal"}]},{"given":"Carlos","family":"Faro","sequence":"additional","affiliation":[{"name":"Biocant\u2013Biotechnology Innovation Center, Parque Tecnol\u00f3gico de Cantanhede, N\u00facleo 4, Lote 8\u20133060-197 Cantanhede, Portugal"},{"name":"CNC\u2013Center for Neuroscience and Cell Biology of Coimbra, University of Coimbra, 3004-517 Coimbra, Portugal"}]},{"given":"Carlos\u00a0F. 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