{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T15:00:49Z","timestamp":1760367649286},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1987,5,1]]},"abstract":"<jats:p>The molybdenum iron-sulphur protein originally isolated from Desulfovibrio gigas by Moura, Xavier, Bruschi, Le Gall, Hall &amp; Cammack [(1976) Biochem. Biophys. Res. Commun. 72, 782-789] has been further investigated by e.p.r. spectroscopy of molybdenum(V). The signal obtained on extended reduction of the protein with sodium dithionite has been shown, by studies at 9 and 35 HGz in 1H2O and 2H2O and computer simulations, to have parameters corresponding to those of the Slow signal from the inactive desulpho form of various molybdenum-containing hydroxylases. Another signal obtained on brief reduction of the protein with small amounts of dithionite was shown by e.p.r. difference techniques to be a Rapid type 2 signal, like that from the active form of such enzymes. In confirmation that the protein is a molybdenum-containing hydroxylase, activity measurements revealed that it had aldehyde:2,6-dichlorophenol-indophenol oxidoreductase activity. No such activity towards xanthine or purine was observed. Salicylaldehyde was a particularly good substrate, and treatment of the protein with it also gave rise to the Rapid signal. Molybdenum cofactor liberated from the protein was active in the nit-1 Neurospora crassa nitrate reductase assay. It is concluded that the protein is a form of an aldehyde oxidase or dehydrogenase. From the intensity of the e.p.r. signals and from enzyme activity measurements, 10-30% of the protein in the sample examined appeared to be in the functional form. The evolutionary significance of the protein, which may represent a primitive form of the enzyme rather than a degradation product, is discussed briefly.<\/jats:p>","DOI":"10.1042\/bj2430755","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:58:17Z","timestamp":1439240297000},"page":"755-761","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["The molybdenum iron-sulphur protein from <i>Desulfovibrio gigas<\/i> as a form of aldehyde oxidase"],"prefix":"10.1042","volume":"243","author":[{"given":"N","family":"Turner","sequence":"first","affiliation":[{"name":"School of Chemistry and Molecular Sciences, University of Sussex, Falmer, Brighton BN1 9QJ, U.K."}]},{"given":"B","family":"Barata","sequence":"additional","affiliation":[{"name":"Centro de Quimica Estrutural U.N.L., 1096 Lisboa-Codex, Portugal GA 30602, U.S.A."}]},{"given":"R C","family":"Bray","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Sciences, University of Sussex, Falmer, Brighton BN1 9QJ, U.K."}]},{"given":"J","family":"Deistung","sequence":"additional","affiliation":[{"name":"School of Chemistry and Molecular Sciences, University of Sussex, Falmer, Brighton BN1 9QJ, U.K."}]},{"given":"J","family":"Le Gall","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Chemistry, University of Georgia, Athens, GA 30602, U.S.A."}]},{"given":"J J G","family":"Moura","sequence":"additional","affiliation":[{"name":"Centro de Quimica Estrutural U.N.L., 1096 Lisboa-Codex, Portugal GA 30602, U.S.A."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/243\/3\/755\/589718\/bj2430755.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/243\/3\/755\/589718\/bj2430755.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T17:49:26Z","timestamp":1637862566000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/243\/3\/755\/23378\/The-molybdenum-iron-sulphur-protein-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1987,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2430755","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,5,1]]}}}