{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:28:49Z","timestamp":1758126529061},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,8,15]]},"abstract":"<jats:p>The sequence of a third member of the Tetrahymenapyriformis chaperonin CCT (\u2018chaperonin containing TCP1\u2019) subunit gene family is presented. This gene, designated TpCCT\u03b1, is the orthologue of the mouse chaperonin gene TCP1\/CCT\u03b1. To characterize the CCT complex in this ciliate, we have produced polyclonal antibodies against synthetic peptides based on C-terminal sequences deduced from the primary sequences of the TpCCT\u03b1, TpCCT\u03b3 and TpCCT\u03b7 subunits. We have also used polyclonal antibodies produced against recombinant yeast CCT\u03b1 and CCT\u03b2 subunits. Using these antibodies, we show that Tetrahymena cells contain a hetero-oligomeric CCT chaperonin comprising at least seven distinct subunits. Three of these were assigned to specific TpCCT genes, whereas a fourth was recognized by the polyclonal antibody against yeast CCT\u03b2, suggesting that this gene is also present in the ciliate. The CCT complex also contains other unidentified proteins that were recognized by the polyclonal antibody UM-1, raised against the putative ATP binding domain of the chaperonin proteins. TpCCT\u03b1 gene expression was shown in exponentially growing cells and cells regenerating their cilia for different periods to have a similar pattern to the previously identified genes TpCCT\u03b3 and TpCCT\u03b7, and also to tubulin genes.<\/jats:p>","DOI":"10.1042\/bj3260021","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:55:50Z","timestamp":1439243750000},"page":"21-29","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The third member of the Tetrahymena CCT subunit gene family, TpCCT\u03b1, encodes a component of the hetero-oligomeric chaperonin complex"],"prefix":"10.1042","volume":"326","author":[{"given":"Helena","family":"SOARES","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto Gulbenkian de Ci\u00eancia, Apartado 14, P-2781 Oeiras codex, Portugal"}]},{"given":"Luisa","family":"CYRNE","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto Gulbenkian de Ci\u00eancia, Apartado 14, P-2781 Oeiras codex, Portugal"},{"name":"Depart. de Quimica e Bioquimica, Faculdade de Ci\u00eancias de Lisboa, Universidade de Lisboa, Lisboa, Portugal"}]},{"given":"Cristina","family":"CASALOU","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto Gulbenkian de Ci\u00eancia, Apartado 14, P-2781 Oeiras codex, Portugal"}]},{"given":"Bruno","family":"EHMANN","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Biologie II\/Botanik, Albert-Ludwigs-Universitaet Freiburg, Schaenzlestrasse 1, D-79104 Freiburg, Germany"}]},{"given":"Claudina","family":"RODRIGUES-POUSADA","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Gen\u00e9tica Molecular, Instituto Gulbenkian de Ci\u00eancia, Apartado 14, P-2781 Oeiras codex, Portugal"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/326\/1\/21\/624309\/bj3260021.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/326\/1\/21\/624309\/bj3260021.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:29:24Z","timestamp":1637713764000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/326\/1\/21\/33761\/The-third-member-of-the-Tetrahymena-CCT-subunit"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3260021","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8,15]]}}}