{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T17:10:09Z","timestamp":1739380209800,"version":"3.37.0"},"reference-count":43,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,10,1]]},"abstract":"<jats:p>UDCA (ursodeoxycholic acid) is used increasingly for the treatment of cholestatic liver diseases. Among other cytoprotective effects, this endogenous bile acid is a potent inhibitor of apoptosis, interfering with both intrinsic and extrinsic apoptotic pathways. In previous studies, we have demonstrated that the transforming growth factor \u03b21-induced E2F-1\/Mdm2 (murine double minute 2)\/p53 apoptotic pathway was an upstream molecular target of UDCA. In agreement with this, we have recently established p53 as a key molecular target in UDCA prevention of cell death. The tumour suppressor p53 is a well-described transcription factor that induces the expression of multiple different pro-apoptotic gene products. Its regulation involves a variety of signalling proteins and small molecules, and occurs at multiple levels, including transcription, translation and post-translation levels. In the present study, by using different biophysical techniques, we have investigated the possibility of a direct interaction between the p53 core domain, also referred to as the DNA-binding domain, and UDCA. Our in vitro analysis did not provide any evidence for direct binding between the bile acid UDCA and the p53 core domain.<\/jats:p>","DOI":"10.1042\/bsr20090107","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T11:34:10Z","timestamp":1255001650000},"page":"359-364","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["No evidence of direct binding between ursodeoxycholic acid and the p53 DNA-binding domain"],"prefix":"10.1042","volume":"30","author":[{"given":"Joana\u00a0D.","family":"Amaral","sequence":"first","affiliation":[{"name":"Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"}]},{"given":"Ana\u00a0R.","family":"Correia","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]},{"given":"Clifford\u00a0J.","family":"Steer","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Minnesota Medical School, Minneapolis, MN, U.S.A."},{"name":"Departments of Genetics, Cell Biology and Development, University of Minnesota Medical School, Minneapolis, MN, U.S.A."}]},{"given":"Cl\u00e1udio\u00a0M.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica, Universidade Nova de Lisboa, Oeiras, Portugal"}]},{"given":"Cec\u00edlia\u00a0M.P.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines and Pharmaceutical Sciences, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal"}]}],"member":"288","published-online":{"date-parts":[[2010,6,3]]},"reference":[{"key":"2021111618064382800_B1","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1038\/nrm2395","article-title":"Transcriptional control of human p53-regulated genes","volume":"9","author":"Riley","year":"2008","journal-title":"Nat. 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