{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T03:31:16Z","timestamp":1773372676924,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":1057,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Clin Investigation"],"published-print":{"date-parts":[[1999,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Activated human lymphocytes were shown to express the elastin\u2013laminin receptor <jats:italic>in vitro<\/jats:italic> and also <jats:italic>in vivo<\/jats:italic> in atherosclerotic plaques. In the presence of the agonist, elastin peptides, this receptor was shown to mediate an increased cell proliferation and an increased synthesis and excretion of an elastase\u2010type serine endopeptidase. In this study, we investigated the variation of the above reaction as a function of agonist concentration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Human lymphocytes were obtained by tonsillectomy and cultured in the presence of phytohaemagglutinin and elastin peptides. Cell viability was evaluated by vital dye exclusion. Elastase and cathepsin G activities were determined in culture supernates and cell lysates using synthetic substrates. Apoptotic cells were identified by the TUNEL method and by electron microscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At increasing concentrations of elastin peptides, a dose\u2010dependent increase in cell death was observed. Up to 100\u2003\u03bcg\u2003mL<jats:sup>\u22121<\/jats:sup> elastin peptides and an increasing fraction of lymphocytes were found permeable to trypan blue, and a large proportion was in apoptosis. Elastin peptide\u2010induced cell death was inhibited by 1\u2003\u03bcg\u2003mL<jats:sup>\u22121<\/jats:sup> lactose and melibiose.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We describe here cell death of human activated lymphocytes expressing the elastin\u2013laminin receptor in the presence of increasing concentrations of elastin peptides, agonists of the receptor. The mechanism of cell death appears to be related to the triggering of the release of elastase and free radicals mediated by the elastin\u2013laminin receptor. Antagonists of this receptor, lactose and melibiose, protected the lymphocytes from the receptor\u2010mediated cell death.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2362.1999.00423.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T15:44:29Z","timestamp":1047397469000},"page":"166-172","source":"Crossref","is-referenced-by-count":49,"title":["Cell death by overload of the elastin\u2013laminin receptor on human activated lymphocytes: protection by lactose and melibiose"],"prefix":"10.1111","volume":"29","author":[{"given":"G.","family":"P\u00e9terszegi","sequence":"first","affiliation":[]},{"given":"S.","family":"Texier","sequence":"additional","affiliation":[]},{"given":"L.","family":"Robert","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,6,11]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.15.5517"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.4.995"},{"key":"e_1_2_5_4_2","first-page":"736","article-title":"Elastonectin and the elastin receptor","volume":"37","author":"Robert L","year":"1989","journal-title":"Pathol Biol"},{"key":"e_1_2_5_5_2","unstructured":"RobertL JacobMP HornebeckW. 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