{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T10:46:47Z","timestamp":1774781207066,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":1096,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:p>We constructed a physical map of the genomic DNA (5.1\u2003Mb) for <jats:italic>Vibrio parahaemolyticus<\/jats:italic> strain AQ4673 by combining 17 adjacent <jats:italic>Not<\/jats:italic>I fragments. This map shows two circular replicons of 3.2 and 1.9\u2003Mb. Pulsed\u2010field gel electrophoresis (PFGE) of undigested genomic DNA revealed two bands of corresponding sizes. Analysis both by <jats:italic>Not<\/jats:italic>I digestion and by Southern blot of the two isolated bands confirmed the existence of two replicons. The presence of genes for 16S rRNA on both the replicons indicates that the replicons are chromosomes rather than megaplasmids. The two bands were also seen after PFGE of undigested genomic DNA of <jats:italic>V. parahaemolyticus<\/jats:italic> strains other than AQ4673, and of strains belonging to other <jats:italic>Vibrio<\/jats:italic> species, such as <jats:italic>V. vulnificus, V. fluvialis<\/jats:italic> and various serovars and biovars of <jats:italic>V. cholerae<\/jats:italic>. It is noteworthy that <jats:italic>V. cholerae<\/jats:italic> O1 strain 569B, a classical biovar, was also shown to have two replicons of 2.9 and 1.2\u2003Mb, which does not agree with a physical map proposed in a previous study. Our results suggest that a two\u2010replicon structure is common throughout <jats:italic>Vibrio<\/jats:italic> species.<\/jats:p>","DOI":"10.1046\/j.1365-2958.1999.01296.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:23:15Z","timestamp":1047468195000},"page":"1513-1521","source":"Crossref","is-referenced-by-count":89,"title":["Physical and genetic map of the genome of <i>Vibrio parahaemolyticus<\/i>: presence of two chromosomes in <i>Vibrio<\/i> 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