{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T20:46:32Z","timestamp":1773866792562,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,7,7]],"date-time":"2008-07-07T00:00:00Z","timestamp":1215388800000},"content-version":"vor","delay-in-days":2440,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p> <jats:italic>Helicobacter pylori<\/jats:italic> colonizes the human stomach and is the causative agent of a variety of gastric diseases. After bacterial attachment, the <jats:italic>H. pylori<\/jats:italic> CagA protein is translocated into gastric epithelial cells and tyrosine phosphorylated. This process is associated with characteristic cytoskeletal rearrangements, resulting in a scatter factor\u2010like (\u2018hummingbird\u2019) phenotype. In this study, using a <jats:italic>cagA<\/jats:italic> mutant complemented with wild\u2010type <jats:italic>cagA<\/jats:italic> and transiently expressing CagA in AGS cells, we have demonstrated that translocated CagA is necessary for rearrangements of the actin cytoskeleton to occur. Anti\u2010phosphotyrosine immunoblotting studies and treatment of infected cells with phosphotyrosine kinase inhibitors suggested that not only translocation but also phosphorylation of CagA is important in this process. Transient expression of CagA\u2013green fluorescent protein (GFP) fusion proteins and two\u2010dimensional gel electrophoresis of CagA protein species demonstrated tyrosine phosphorylation in the C\u2010terminus. Site\u2010directed mutagenesis of CagA revealed that tyrosine residue 972 is essential for induction of the cellular phenotype. We have also demonstrated that translocation and phosphorylation of CagA is necessary but not sufficient for induction of the hummingbird phenotype in AGS cells, indicating the involvement of as yet unidentified bacterial factor(s).<\/jats:p>","DOI":"10.1046\/j.1365-2958.2001.02649.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:40:58Z","timestamp":1047469258000},"page":"631-644","source":"Crossref","is-referenced-by-count":198,"title":["Phosphorylation of tyrosine 972 of the <i>Helicobacter pylori<\/i> CagA protein is essential for induction of a scattering phenotype in gastric epithelial 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