{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T18:42:27Z","timestamp":1770748947649,"version":"3.50.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,7,7]],"date-time":"2008-07-07T00:00:00Z","timestamp":1215388800000},"content-version":"vor","delay-in-days":2440,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p>Efficient uptake of <jats:italic>Yersinia pseudotuberculosis<\/jats:italic> into cultured mammalian cells is the result of high\u2010affinity binding of invasin to \u03b2<jats:sub>1<\/jats:sub> chain integrins. We demonstrate here that uptake requires Rac1 and Arp 2\/3 function. Bacterial uptake was stimulated by GTP\u03b3S, but was inhibited in mammalian cells transfected with the interfering Rac1\u2010N17 derivative. Rac1 was found to be activated in response to integrin engagement by invasin, whereas Rac1 and Arp 2\/3 were found to be intensely localized around phagosomes bearing bacteria, indicating a specific role for Rac1 signalling from the nascent phagosome to downstream effectors. To determine whether the Arp 2\/3 complex was a component of this proposed pathway, cells overproducing various derivatives of Scar1\/WAVE1, an Arp 2\/3\u2010binding protein, were analysed. Sequestration of Arp 2\/3 away from the phagocytic cup as a result of Scar1\/WAVE1 overproduction dramatically inhibited uptake. To determine whether signalling from Rac1 to Arp 2\/3 occurred via N\u2010WASP, uptake was analysed in a cell line lacking expression of WASP and N\u2010WASP. Uptake was unaffected by the absence of these proteins, indicating that \u03b2<jats:sub>1<\/jats:sub> integrin signalling from Rac1 to Arp 2\/3 can occur in the absence of N\u2010WASP function.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2001.02676.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:40:58Z","timestamp":1047469258000},"page":"689-703","source":"Crossref","is-referenced-by-count":79,"title":["Efficient uptake of <i>Yersinia pseudotuberculosis<\/i> via integrin receptors involves a Rac1\u2013Arp 2\/3 pathway that bypasses N\u2010WASP function"],"prefix":"10.1111","volume":"42","author":[{"given":"Michael 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