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Yeast cells and cell wall proteins (CWP) of the human pathogenic fungus <jats:italic>Candida albicans<\/jats:italic> bound plasminogen with a <jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub> of 70\u2003\u00b1\u200311\u2003nM and 112\u2003\u00b1\u200320\u2003nM respectively. Bound plasminogen could be activated to plasmin by mammalian plasminogen activators; no <jats:italic>C. albicans<\/jats:italic> plasminogen activator was detected. Binding of plasminogen to CWP and whole cells was inhibited by \u025bACA, indicating that binding was predominantly to lysine residues. <jats:italic>Candida albicans<\/jats:italic> mutant strains defective in protein glycosylation did not show altered plasminogen binding, suggesting that binding was not mediated via a surface lectin. Binding was sensitive to digestion by basic carboxypeptidase, implicating C\u2010terminal lysine residues in binding. Proteomic analysis identified eight major plasminogen\u2010binding proteins in isolated CWP. Five of these (phosphoglycerate mutase, alcohol dehydrogenase, thioredoxin peroxidase, catalase, transcription elongation factor) had C\u2010terminal lysine residues and three (glyceraldehyde\u20103\u2010phosphate dehydrogenase, phosphoglycerate kinase and fructose bisphosphate aldolase) did not. Activation of plasminogen could potentially increase the capacity of this pathogenic fungus for tissue invasion and necrosis. Although surface\u2010bound plasmin(ogen) degraded fibrin, no direct evidence for a role in invasion of endothelial matrix or in penetration and damage of endothelial cells was found.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2003.03390.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:47:46Z","timestamp":1047469666000},"page":"1637-1651","source":"Crossref","is-referenced-by-count":208,"title":["<i>Candida albicans<\/i> binds human plasminogen: identification of eight plasminogen\u2010binding proteins"],"prefix":"10.1111","volume":"47","author":[{"given":"Jonathan D.","family":"Crowe","sequence":"first","affiliation":[]},{"given":"Isla K.","family":"Sievwright","sequence":"additional","affiliation":[]},{"given":"Gillian C.","family":"Auld","sequence":"additional","affiliation":[]},{"given":"Norma R.","family":"Moore","sequence":"additional","affiliation":[]},{"given":"Neil A. 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