{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:06:39Z","timestamp":1760897199549},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":724,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neuropathology Appl Neurobio"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Permanent glioma cell lines are invaluable tools in understanding the biology of glioblastomas. The present study reports the establishment of a clonal human cell line, GBM6840, derived from a biopsy of paediatric cerebellar glioblastoma multiforme. GBM6840 had a doubling time of 32\u2003h and grew as a monolayer of large round cells that retained immunopositivity for glial fibrillary acidic protein and vimentin. Karyotypic analysis revealed a modal chromosome number of 68 and polysomies of chromosomes 3, 5 and 20, as well as the presence of 3\u20134 marker chromosomes. GBM6840 also showed anchorage\u2010independent growth in soft agar and tumour formation in nude mice. The <jats:italic>p16<\/jats:italic>(<jats:italic>CDKN2A<\/jats:italic>) gene was transcriptionally silenced by hypermethylation, consistent with the lack of protein expression observed in the original tumour and cultured cells. Western blot analysis revealed normal protein expression of pRb and CDK4. It appears that p16 is the major component altered in the cell cycle pathway and may confer these cells unrestrained proliferation potential. Neither <jats:italic>EGFR<\/jats:italic> gene amplification nor over\u2010expression of the protein was detected in the cultured cells. Over\u2010expression of the p53 protein was observed in the majority of cells, despite undetectable mutation (exons 5\u20138) in the gene. One allele of the <jats:italic>PTEN<\/jats:italic> gene was found to be mutated during <jats:italic>in vitro<\/jats:italic> cultivation. Telomerase activity was demonstrated in the cultured cells but not in the original tumour, supporting the hypothesis that telomerase is required for the <jats:italic>in vitro<\/jats:italic> immortalization process.<\/jats:p>","DOI":"10.1046\/j.1365-2990.2000.00214.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T14:51:30Z","timestamp":1047480690000},"page":"22-30","source":"Crossref","is-referenced-by-count":14,"title":["Establishment and characterization of a human cell line from paediatric cerebellar glioblastoma multiforme"],"prefix":"10.1111","volume":"26","author":[{"given":"E.","family":"Di Tomaso","sequence":"first","affiliation":[]},{"given":"J. C. S.","family":"Pang","sequence":"additional","affiliation":[]},{"given":"H. K.","family":"Ng","sequence":"additional","affiliation":[]},{"given":"P. Y. P.","family":"Lam","sequence":"additional","affiliation":[]},{"given":"X. 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