{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T01:37:31Z","timestamp":1780537051636,"version":"3.54.1"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,1,5]],"date-time":"2002-01-05T00:00:00Z","timestamp":1010188800000},"content-version":"vor","delay-in-days":1434,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[1998,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The tomato yellow leaf curl virus (TYLCV) found in Israel is a whitefly\u2010transmitted monopartite geminivirus. Although geminiviruses have been found in the nuclei of phloem\u2010associated cells, the mechanism of viral invasion is poorly understood. The possible role of the TYLCV capsid protein (CP), the only known component of the viral coat, in virus transport into the host cell nucleus was investigated by monitoring its specific nuclear accumulation in plant and insect cells. CP was fused to the \u03b2\u2010glucuronidase (GUS) reporter enzyme to assay nuclear import in petunia protoplasts, and micro\u2010injection of purified fluorescently labeled CP was used to examine its nuclear uptake in <jats:italic>Drosophila<\/jats:italic> embryos. Both assays demonstrated that TYLCV CP is transported into plant\u2010and insect\u2010cell nuclei by an active process of nuclear import via a nuclear localization signal (NLS)\u2010specific pathway. Using the GUS assay and deletion analysis, the TYLCV CP NLS sequence was identified in the amino\u2010terminus of the protein.<\/jats:p>","DOI":"10.1046\/j.1365-313x.1998.00037.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:52:02Z","timestamp":1047505922000},"page":"393-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Nuclear import of the capsid protein of tomato yellow leaf curl virus (TYLCV) in plant and insect cells"],"prefix":"10.1111","volume":"13","author":[{"given":"Talya","family":"Kunik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karuppaiah","family":"Palanichelvam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henryk","family":"Czosnek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaly","family":"Citovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yedidya","family":"Gafni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,5]]},"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-313X.1998.00037.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-313X.1998.00037.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T19:47:37Z","timestamp":1694375257000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-313X.1998.00037.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,2]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1998,2]]}},"alternative-id":["10.1046\/j.1365-313X.1998.00037.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-313x.1998.00037.x","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,2]]},"assertion":[{"value":"2002-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}