{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T06:00:22Z","timestamp":1775800822248,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,5]],"date-time":"2002-01-05T00:00:00Z","timestamp":1010188800000},"content-version":"vor","delay-in-days":1253,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:bold>The green fluorescent protein (GFP) from<\/jats:bold>\n          <jats:italic>\n            <jats:bold>Aequorea victoria<\/jats:bold>\n          <\/jats:italic>\n          <jats:bold>can be detected in living plant cells after transient transformation of protoplasts. Expression of the GFP can be used to monitor protein trafficking in a mixed cell population and also to study the different function and importance of organelles in different cell types. We developed a vacuolar form of GFP that was obtained by replacing the C\u2010terminal endoplasmic reticulum (ER)\u2010retention motif of mGFP5\u2010ER by the vacuolar targeting peptide of tobacco chitinase A. The vacuolar GFP was transported and accumulated in the vacuole as expected. However, we found two patterns of GFP accumulation after prolonged incubation (18\u201324\u2003h) depending on the cell type. Most chloroplast\u2010rich protoplasts had a fluorescent large central vacuole. In contrast, most chloroplast\u2010poor protoplasts accumulated the GFP in one smaller vacuole but not in the large central vacuole, which was visible under a light microscope in the same cell. This differential accumulation reflected the existence of two different vacuolar compartments as described recently by immunolocalization of several vacuolar markers. We were able to characterize the vacuolar compartment to which GFP is specifically targeted as non\u2010acidic, since it did not accumulate neutral red while acidic vacuoles did not accumulate GFP.<\/jats:bold> <\/jats:p>","DOI":"10.1046\/j.1365-313x.1998.00210.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:54:18Z","timestamp":1047506058000},"page":"449-457","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":138,"title":["<b>Specific accumulation of GFP in a non\u2010acidic vacuolar compartment via a C\u2010terminal propeptide\u2010mediated sorting pathway<\/b>"],"prefix":"10.1111","volume":"15","author":[{"given":"Gian\u2010Pietro","family":"Di Sansebastiano","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadine","family":"Paris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Marc\u2010Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Marc","family":"Neuhaus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1996.10050935.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.5.5.587"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.pcp.a029007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1007\/BF00202644","article-title":"Kinetics of prolyl hydroxylation, intracellular transport and C\u2010terminal processing of the tobacco vacuolar chitinase.","volume":"147","author":"Freydl E.","year":"1995","journal-title":"Planta"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.5.9.1113"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.2307\/3869425","article-title":"Endoplasmic reticulum forms a dynamic continuum for lipid diffusion between contiguous soybean root cells.","volume":"5","author":"Grabski S.","year":"1993","journal-title":"Plant Cell"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2122"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12501"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"2539","DOI":"10.1242\/jcs.109.10.2539","article-title":"Transport of storage proteins to the vacuole is mediated by vesicles without a clathrin coat.","volume":"109","author":"Hohl I.","year":"1996","journal-title":"J. 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Bot."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00015814"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1994.5010045.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006032627036"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.22.10362"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.47.1.327"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81256-8"},{"key":"e_1_2_6_22_1","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.101.2.451"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)70789-6"}],"container-title":["The Plant Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-313X.1998.00210.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-313X.1998.00210.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1046\/j.1365-313X.1998.00210.x","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-313X.1998.00210.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T01:54:57Z","timestamp":1694397297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-313X.1998.00210.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,8]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,8]]}},"alternative-id":["10.1046\/j.1365-313X.1998.00210.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-313x.1998.00210.x","archive":["Portico"],"relation":{},"ISSN":["0960-7412","1365-313X"],"issn-type":[{"value":"0960-7412","type":"print"},{"value":"1365-313X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,8]]},"assertion":[{"value":"2002-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}