{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T04:10:15Z","timestamp":1773461415055,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":1181,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p> <jats:bold>The Arabidopsis PR\u20101 gene is one of a suite of genes induced co\u2010ordinately during the onset of systemic acquired resistance (SAR), a plant defense pathway triggered by pathogen infection or exogenous application of chemicals such as salicylic acid (SA) and 2,6\u2010dichloroisonicotinic acid (INA). We have characterized<\/jats:bold>\n          <jats:italic>\n            <jats:bold>cis<\/jats:bold>\n          <\/jats:italic>\n          <jats:bold>\u2010acting regulatory elements in the PR\u20101 promoter involved in INA induction using deletion analysis, linker\u2010scanning mutagenesis, and<\/jats:bold>\n          <jats:italic>\n            <jats:bold>in\u2003vivo<\/jats:bold>\n          <\/jats:italic>\n          <jats:bold>footprinting. Compared to promoter fragments of 815\u2003bp or longer (which show greater than 10\u2010fold inducibility after INA treatment), induction of a 698\u2003bp long promoter fragment is reduced by half and promoter fragments of 621\u2003bp or shorter have lost all inducibility. Additionally, two 10\u2010bp linker\u2010scanning mutations centered at 640\u2003bp and 610\u2003bp upstream from the transcription initiation site are each sufficient to abolish chemical inducibility of a GUS reporter fusion. The \u2013640 linker\u2010scanning mutation encompasses a region highly homologous to recognition sites for transcription factors of the basic leucine zipper class, while the \u2013610 linker\u2010scanning mutation contains a sequence similar to a consensus recognition site for the transcription factor NF\u2010\u03baB. Furthermore, several inducible<\/jats:bold>\n          <jats:italic>\n            <jats:bold>in\u2003vivo<\/jats:bold>\n          <\/jats:italic>\n          <jats:bold>footprints located at or nearby these motifs demonstrate significant and highly reproducible changes in DNA accessibility following SAR induction. This<\/jats:bold>\n          <jats:italic>\n            <jats:bold>in\u2003vivo<\/jats:bold>\n          <\/jats:italic>\n          <jats:bold>signature of protein\u2013DNA interactions after INA induction is tightly correlated with the functionally important regions of the promoter identified by mutation analysis.<\/jats:bold> <\/jats:p>","DOI":"10.1046\/j.1365-313x.1998.00288.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T21:55:21Z","timestamp":1047506121000},"page":"223-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":282,"title":["<b>Functional analysis of regulatory sequences controlling<\/b><b>PR\u20101 gene expression in Arabidopsis<\/b>"],"prefix":"10.1111","volume":"16","author":[{"given":"Edouard","family":"Lebel","sequence":"first","affiliation":[]},{"given":"Peter","family":"Heifetz","sequence":"additional","affiliation":[]},{"given":"Leigh","family":"Thorne","sequence":"additional","affiliation":[]},{"given":"Scott","family":"Uknes","sequence":"additional","affiliation":[]},{"given":"John","family":"Ryals","sequence":"additional","affiliation":[]},{"given":"Eric","family":"Ward","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0885-5765(92)90045-W"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.8.1.15"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81318-5"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001041"},{"key":"e_1_2_6_6_1","first-page":"1194","article-title":"In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants.","volume":"316","author":"Bechtold N.","year":"1993","journal-title":"C. 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