{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T19:09:01Z","timestamp":1776712141688,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":542,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To investigate the uptake and long\u2010distance translocation of sulphate in plants, we have characterized three cell\u2010type\u2010specific sulphate transporters, <jats:italic>Sultr1;1<\/jats:italic>, <jats:italic>Sultr2;1<\/jats:italic> and <jats:italic>Sultr2;2<\/jats:italic> in <jats:italic>Arabidopsis thaliana<\/jats:italic>. Heterologous expression in the yeast sulphate transporter mutant indicated that <jats:italic>Sultr1;1<\/jats:italic> encodes a high\u2010affinity sulphate transporter (<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> for sulphate 3.6\u2003\u00b1\u20030.6\u2003\u03bc<jats:sc>m<\/jats:sc>), whereas <jats:italic>Sultr2;1<\/jats:italic> and <jats:italic>Sultr2;2<\/jats:italic> encode low\u2010affinity sulphate transporters (<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub> for sulphate 0.41\u2003\u00b1\u20030.07\u2003m\n\t\t\t\t\t<jats:sc>m<\/jats:sc> and \u2265\u200a1.2\u2003m\n\t\t\t\t\t<jats:sc>m<\/jats:sc>, respectively). In <jats:italic>Arabidopsis<\/jats:italic> plants expressing the fusion gene construct of the <jats:italic>Sultr1;1<\/jats:italic> promoter and green fluorescent protein (GFP), GFP was localized in the lateral root cap, root hairs, epidermis and cortex of roots. \u03b2\u2010glucuronidase (GUS) expressed with the <jats:italic>Sultr2;1<\/jats:italic> promoter was specifically accumulated in the xylem parenchyma cells of roots and leaves, and in the root pericycles and leaf phloem. Expression of the <jats:italic>Sultr2;2<\/jats:italic> promoter\u2013GFP fusion gene showed specific localization of GFP in the root phloem and leaf vascular bundle sheath cells. Plants continuously grown with low sulphate concentrations accumulated high levels of <jats:italic>Sultr1;1<\/jats:italic> and <jats:italic>Sultr2;1<\/jats:italic> mRNA in roots and <jats:italic>Sultr2;2<\/jats:italic> mRNA in leaves. The abundance of <jats:italic>Sultr1;1<\/jats:italic> and <jats:italic>Sultr2;1<\/jats:italic> mRNA was increased remarkably in roots by short\u2010term stress caused by withdrawal of sulphate. Addition of selenate in the sulphate\u2010sufficient medium increased the sulphate uptake capacity, tissue sulphate content and the abundance of <jats:italic>Sultr1;1<\/jats:italic> and <jats:italic>Sultr2;1<\/jats:italic> mRNA in roots. Concomitant decrease of the tissue thiol content after selenate treatment was consistent with the suggested role of glutathione (GSH) as a repressive effector for the expression of sulphate transporter genes.<\/jats:p>","DOI":"10.1046\/j.1365-313x.2000.00768.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T22:00:58Z","timestamp":1047506458000},"page":"171-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":516,"title":["The roles of three functional sulphate transporters involved in uptake and translocation of sulphate in <i>Arabidopsis thaliana<\/i>"],"prefix":"10.1111","volume":"23","author":[{"given":"Hideki","family":"Takahashi","sequence":"first","affiliation":[]},{"given":"Akiko","family":"Watanabe\u2010Takahashi","sequence":"additional","affiliation":[]},{"given":"Frank W.","family":"Smith","sequence":"additional","affiliation":[]},{"given":"Mechteld","family":"Blake\u2010Kalff","sequence":"additional","affiliation":[]},{"given":"Malcolm J.","family":"Hawkesford","sequence":"additional","affiliation":[]},{"given":"Kazuki","family":"Saito","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00263858"},{"key":"e_1_2_6_3_1","volume-title":"Current Protocols in Molecular Biology.","author":"Ausubel F.M.","year":"1994"},{"key":"e_1_2_6_4_1","first-page":"1194","article-title":"In planta Agobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants","volume":"316","author":"Bechtold N.","year":"1993","journal-title":"C. 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