{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T07:29:51Z","timestamp":1768289391314,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,3,26]],"date-time":"2002-03-26T00:00:00Z","timestamp":1017100800000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Plant Journal"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>fab1<\/jats:italic> mutant of <jats:italic>Arabidopsis<\/jats:italic> is partially deficient in activity of <jats:italic>\u03b2<\/jats:italic>\u2010ketoacyl\u2010[acyl carrier protein] synthase II (KAS II). This defect results in increased levels of 16\u2003:\u20030 fatty acid and is associated with damage and death of the mutants at low temperature. Transformation of <jats:italic>fab1<\/jats:italic> plants with a cDNA from <jats:italic>Brassica napus<\/jats:italic> encoding a KAS II enzyme resulted in complementation of both mutant phenotypes. The dual complementation by expression of the single gene proves that low\u2010temperature damage is a consequence of altered membrane unsaturation. The <jats:italic>fab1<\/jats:italic> mutation is a single nucleotide change in <jats:italic>Arabidopsis KAS2<\/jats:italic> that results in a Leu337Phe substitution. The Leu337 residue is conserved among plant and bacterial KAS proteins, and in the crystal structures of <jats:italic>E.\u2003coli<\/jats:italic> KAS I and KAS II, this leucine abuts a phenylalanine whose imidazole ring extends into the substrate binding cavity causing the fatty acid chain to bend. For functional analysis the equivalent Leu207Phe mutation was introduced into the <jats:italic>fabB<\/jats:italic> gene encoding the <jats:italic>E.\u2003coli<\/jats:italic> KAS I enzyme. Compared to wild\u2010type, the Leu207Phe protein showed a 10\u2010fold decrease in binding affinity for the fatty acid substrate, exhibited a modified behavior during size\u2010exclusion chromatography and was severely impaired in condensation activity. These results suggest that the molecular defect in <jats:italic>fab1<\/jats:italic> plants is a structural instability of the <jats:italic>KAS2<\/jats:italic> gene product induced by insufficient space for the imidazole ring of the mutant phenylalanine residue.<\/jats:p>","DOI":"10.1046\/j.1365-313x.2002.01253.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T22:06:26Z","timestamp":1047506786000},"page":"761-770","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["A <i>KAS2<\/i> cDNA complements the phenotypes of the <i>Arabidopsis fab1<\/i> mutant that differs in a single residue bordering the substrate binding pocket"],"prefix":"10.1111","volume":"29","author":[{"given":"Anders S.","family":"Carlsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel T.","family":"LaBrie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Kinney","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Penny","family":"Von Wettstein\u2010Knowles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Browse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02670471"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.9.3628"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.22.8711"},{"key":"e_1_2_6_5_1","unstructured":"Browse J. 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