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To decipher the molecular mechanisms and the predicted complex network of regulatory pathways involved in the senescence program, we have carried out a large\u2010scale gene identification study in a reference plant, <jats:italic>Arabidopsis thaliana<\/jats:italic>. Using suppression subtractive hybridization, we isolated approximately 800 cDNA clones representing SAGs expressed in senescing leaves. Differential expression was confirmed by Northern blot analysis for 130 non\u2010redundant genes. Over 70 of the identified genes have not previously been shown to participate in the senescence process. SAG\u2010encoded proteins are likely to participate in macromolecule degradation, detoxification of oxidative metabolites, induction of defense mechanisms, and signaling and regulatory events. Temporal expression profiles of selected genes displayed several distinct patterns, from expression at a very early stage, to the terminal phase of the senescence syndrome. Expression of some of the novel SAGs, in response to age, leaf detachment, darkness, and ethylene and cytokinin treatment was compared. The large repertoire of SAGs identified here provides global insights about regulatory, biochemical and cellular events occurring during leaf senescence.<\/jats:p>","DOI":"10.1046\/j.1365-313x.2003.01908.x","type":"journal-article","created":{"date-parts":[[2003,11,13]],"date-time":"2003-11-13T23:57:47Z","timestamp":1068767867000},"page":"629-642","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":318,"title":["Large\u2010scale identification of leaf senescence\u2010associated genes"],"prefix":"10.1111","volume":"36","author":[{"given":"Shimon","family":"Gepstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gazalah","family":"Sabehi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Jeanne","family":"Carp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taleb","family":"Hajouj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizied Falah Orna","family":"Nesher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inbal","family":"Yariv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Dor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Bassani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,11,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.012732"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004250000423"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(02)02284-7"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(02)00298-9"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.105.3.839"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/48.2.181"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005774212410"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1467-7652.2003.00004.x"},{"issue":"1","key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1042\/bj2910193","article-title":"Metallothionein protects DNA from oxidative damage","volume":"291","author":"Chubatsu L.S.","year":"1993","journal-title":"Biochem. 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