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A novel method was developed in which mutacin 1140 was chemically modified with sodium borohydride followed by ethanethiol, allowing the differentiation of the thioether\u2010containing residues from the dehydrated residues. This double\u2010labeling strategy provides a simple method to\u00a0reliably identify all modified lantibiotic residues with a minimal amount of material. While NMR spectroscopy\u00a0is still required to obtain thioether bridging patterns and thus the complete covalent structure, the double\u2010labeling technique, along with mass spectrometry, provides most of the information in a fraction of the time required for a complete NMR analysis. Thus, with these new techniques lantibiotics can be rapidly characterized.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2000.01777.x","type":"journal-article","created":{"date-parts":[[2003,8,20]],"date-time":"2003-08-20T07:48:59Z","timestamp":1061365739000},"page":"6810-6816","source":"Crossref","is-referenced-by-count":36,"title":["Covalent structure of mutacin 1140 and a novel method for the rapid identification of lantibiotics"],"prefix":"10.1111","volume":"267","author":[{"given":"Leif","family":"Smith","sequence":"first","affiliation":[]},{"given":"Jan","family":"Nov\u00e1k","sequence":"additional","affiliation":[]},{"given":"Jim","family":"Rocca","sequence":"additional","affiliation":[]},{"given":"Scott","family":"McClung","sequence":"additional","affiliation":[]},{"given":"J. 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