{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:42:36Z","timestamp":1773301356819,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2002,7,3]],"date-time":"2002-07-03T00:00:00Z","timestamp":1025654400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:p>The purified soluble forms of the histidine kinases BvgS and EvgS of <jats:italic>Bordetella pertussis<\/jats:italic> and <jats:italic>Escherichia coli<\/jats:italic>, respectively, are shown to be responsive to oxidized ubiquinone\u20100 (Q\u20100) <jats:italic>in vitro<\/jats:italic>. The oxidized ubiquinone is a strong inhibitor of kinase activity of both enzymes with half maximal inhibition occurring at 11\u2003\u00b5<jats:sc>m<\/jats:sc> (BvgS) and 4\u2003\u00b5<jats:sc>m<\/jats:sc> (EvgS). Reduced Q\u20100 has no effect on the histidine kinases. Kinase activity can reversibly be switched off and on by changing the oxidation status of the quinone. This inhibitory effect is due to a decrease of the kinase activity of BvgS rather than an increase of intrinsic phosphatase activities. Other electron carriers such as menadione (MK\u20103), NAD or FAD did not have a significant effect on the kinase activities of BvgS and EvgS. Nicotinic acid and sulfate ions, known to inhibit the histidine kinases <jats:italic>in vivo<\/jats:italic>, did not affect the purified truncated sensor proteins lacking their periplasmic domains <jats:italic>in vitro<\/jats:italic>. Mutations introduced by site\u2010directed mutagenesis into the putative PAS domain of BvgS caused a weak decrease of qui\u2010 none\u2010dependent inhibition of autophosphorylation. These data suggest that BvgS and EvgS are connected with the oxi\u2010 dation status of the cell via the link to the ubiquinone pool.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2002.03029.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:32:52Z","timestamp":1047407572000},"page":"3479-3484","source":"Crossref","is-referenced-by-count":60,"title":["The unorthodox histidine kinases BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier"],"prefix":"10.1111","volume":"269","author":[{"given":"Andreas","family":"Bock","sequence":"first","affiliation":[]},{"given":"Roy","family":"Gross","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,7,3]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.110.10.1141"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(99)01458-4"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00109"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.519"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1455-1458.2001"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1995.0245"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00716.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.3.1163"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90733-1"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.52.33176"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.43.1.263-269.1984"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.3.807-815.1993"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.2.479-506.1999"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02173217"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)90870-2"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.3.970-979.1992"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2508(96)80282-8"},{"key":"e_1_2_7_19_2","first-page":"171","article-title":"Phase\u2010shift markers in the genus Bordetella: loss of cytochrome d\u2010629 in phase IV variants","volume":"31","author":"Ezzell J.W.","year":"1981","journal-title":"Microbios"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-002-0685-2"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(99)80032-0"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(97)00034-0"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1059361"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2001.4773"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1996.tb02636.x"},{"key":"e_1_2_7_26_2","doi-asserted-by":"crossref","first-page":"9441","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli, U.K.","year":"1970","journal-title":"Nature"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.3509"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-4-1257"},{"key":"e_1_2_7_29_2","first-page":"642","volume-title":"Escherichia coli and Salmonella typhimurium","author":"Meganathan R.","year":"1996"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100118697"},{"key":"e_1_2_7_31_2","first-page":"217","volume-title":"Escherichia Coli and Salmonella Typhimurium","author":"Gennis R.B.","year":"1996"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251408398"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.24.7206-7212.2001"},{"key":"e_1_2_7_34_2","doi-asserted-by":"crossref","first-page":"2762","DOI":"10.1128\/IAI.66.6.2762-2768.1998","article-title":"Neither the Bvg\u2010 phase nor the vrg6 locus of Bordetella pertussis isrequired for respiratory infection in mice","volume":"66","author":"Martinez de Tejada G.","year":"1998","journal-title":"Infect. 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