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Here, we demonstrate <jats:italic>in vivo<\/jats:italic> that the high potential iron\u2010sulfur protein (HiPIP) from <jats:italic>Allochromatium vinosum<\/jats:italic> is translocated into the periplasmic space by the Tat system of <jats:italic>Escherichia coli<\/jats:italic>. <jats:italic>In vitro<\/jats:italic>, reconstituted HiPIP precursor (preHoloHiPIP) was targeted to inverted membrane vesicles from <jats:italic>E.\u2003coli<\/jats:italic> by a process requiring ATP when the Tat substrate was properly folded. During membrane targeting, the protein retained its cofactor, indicating that it was targeted in a folded state. Membrane targeting did not require a twin arginine motif and known Tat system components. On the basis of these findings, we propose that a pathway exists for the insertion of folded cofactor\u2010containing proteins such as HiPIP into the bacterial cytoplasmic membrane.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2003.03481.x","type":"journal-article","created":{"date-parts":[[2004,12,23]],"date-time":"2004-12-23T02:53:30Z","timestamp":1103770410000},"page":"1211-1221","source":"Crossref","is-referenced-by-count":61,"title":["Membrane targeting of a folded and cofactor\u2010containing protein"],"prefix":"10.1111","volume":"270","author":[{"given":"Thomas","family":"Br\u00fcser","sequence":"first","affiliation":[]},{"given":"Takahiro","family":"Yano","sequence":"additional","affiliation":[]},{"given":"Daniel C.","family":"Brune","sequence":"additional","affiliation":[]},{"given":"Fevzi","family":"Daldal","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,2,24]]},"reference":[{"key":"e_1_2_25_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05480.x"},{"key":"e_1_2_25_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01719.x"},{"key":"e_1_2_25_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(00)66028-2"},{"key":"e_1_2_25_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35073038"},{"key":"e_1_2_25_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000800200"},{"key":"e_1_2_25_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.51.36073"},{"key":"e_1_2_25_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02263.x"},{"key":"e_1_2_25_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100682200"},{"key":"e_1_2_25_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.11591"},{"key":"e_1_2_25_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02428-0"},{"key":"e_1_2_25_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.11.2982"},{"key":"e_1_2_25_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.13.3851"},{"key":"e_1_2_25_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13106.x"},{"key":"e_1_2_25_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000833200"},{"key":"e_1_2_25_16_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02253.x"},{"key":"e_1_2_25_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.19.13223"},{"key":"e_1_2_25_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00174-2"},{"key":"e_1_2_25_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02514.x"},{"key":"e_1_2_25_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.10.2472"},{"key":"e_1_2_25_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201711200"},{"key":"e_1_2_25_22_2","doi-asserted-by":"crossref","first-page":"4212","DOI":"10.1016\/S0021-9258(19)42505-2","article-title":"Two\u2010Angstrom crystal structure of oxidized Chromatium high potential iron protein","volume":"249","author":"Carter C.W.","year":"1974","journal-title":"J. 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