{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T15:09:52Z","timestamp":1769180992681,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2003,5,8]],"date-time":"2003-05-08T00:00:00Z","timestamp":1052352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:p>The toxicity of the pea albumin 1b (PA1b), a 37 amino\u2010acid peptide extracted from pea seeds, for cereal weevils (<jats:italic>Sitophilus\u2003oryzae<\/jats:italic>, <jats:italic>Sitophilus\u2003granarius<\/jats:italic> and <jats:italic>Sitophilus\u2003zeamais<\/jats:italic>) was recently discovered. The mechanism of action of this new entomotoxin is still unknown and potentially involves a target protein in the insect tissues. This work describes the characterization of a high\u2010affinity binding site for PA1b in a microsomal fraction of <jats:italic>Sitophilus<\/jats:italic> spp. extracts. Purified PA1b was labeled to a high specific radioactivity (<jats:italic>c<\/jats:italic>. 900\u2003Ci\u00b7mmol<jats:sup>\u22121<\/jats:sup>) using <jats:sup>125<\/jats:sup>I, and the iodinated ligand was found to be biologically active. Binding of this ligand to the microsomal fraction of <jats:italic>S.\u2003oryzae<\/jats:italic> extract was found to be saturable and reversible, with an affinity (<jats:italic>K<\/jats:italic><jats:sub>d<\/jats:sub>) of 2.6\u2003n<jats:sc>m<\/jats:sc>, and a high maximal binding capacity (<jats:italic>B<\/jats:italic><jats:sub>max<\/jats:sub>) of 40\u2003pmol\u00b7mg<jats:sup>\u22121<\/jats:sup> of protein. A binding site displaying similar characteristics was detectable in the five susceptible weevils strains tested, as well as in the pea aphid or in the fruit fly. However, no binding activity was detectable in extracts from four <jats:italic>S.\u2003oryzae<\/jats:italic> strains previously shown to be resistant to the toxin through a recessive monogenic mechanism. Therefore, we suggest that this binding site might be involved in the mechanism of action of PA1b.<\/jats:p>","DOI":"10.1046\/j.1432-1033.2003.03611.x","type":"journal-article","created":{"date-parts":[[2003,5,20]],"date-time":"2003-05-20T14:34:23Z","timestamp":1053441263000},"page":"2429-2435","source":"Crossref","is-referenced-by-count":39,"title":["Characterization of a high\u2010affinity binding site for the pea albumin 1b entomotoxin in the weevil <i>Sitophilus<\/i>"],"prefix":"10.1111","volume":"270","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Gressent","sequence":"first","affiliation":[]},{"given":"Isabelle","family":"Rahioui","sequence":"additional","affiliation":[]},{"given":"Yvan","family":"Rahb\u00e9","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2003,5,8]]},"reference":[{"key":"e_1_2_21_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.62.3.775-806.1998"},{"key":"e_1_2_21_3_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.2.347"},{"key":"e_1_2_21_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9063(199802)52:2<165::AID-PS679>3.0.CO;2-7"},{"key":"e_1_2_21_5_2","unstructured":"Delobel B. Grenier A. Gueguen J. Ferrasson E.&Mbailao M.(1998) Utilisation D'un Polypeptide D\u00e9riv\u00e9 D'une Albumine PA1b de L\u00e9gumineuse Comme Insecticide. French Patent 98 05877."},{"key":"e_1_2_21_6_2","doi-asserted-by":"crossref","first-page":"11124","DOI":"10.1016\/S0021-9258(18)67357-0","article-title":"Gene structure, protein structure and regulation of the synthesis of a sulfur\u2010rich protein in pea seeds","volume":"261","author":"Higgins T.J.V.","year":"1986","journal-title":"J. Biol. Chem."},{"key":"e_1_2_21_7_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19940090"},{"key":"e_1_2_21_8_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN19880096"},{"key":"e_1_2_21_9_2","first-page":"232","volume-title":"Proceedings of the XXth International Conference on Magnetic Resonance in Biological Systems","author":"Jouvensal L.","year":"2002"},{"key":"e_1_2_21_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90127-C"},{"key":"e_1_2_21_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(00)00160-4"},{"key":"e_1_2_21_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.191366898"},{"key":"e_1_2_21_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.hdy.6881730"},{"key":"e_1_2_21_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb20008.x"},{"key":"e_1_2_21_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.45.1.371"},{"key":"e_1_2_21_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(95)00102-6"},{"key":"e_1_2_21_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(98)00027-7"},{"key":"e_1_2_21_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01480-X"},{"key":"e_1_2_21_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00149-4"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1432-1033.2003.03611.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1432-1033.2003.03611.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T23:18:04Z","timestamp":1695683884000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1046\/j.1432-1033.2003.03611.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,8]]},"references-count":18,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1046\/j.1432-1033.2003.03611.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1432-1033.2003.03611.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,8]]}}}