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Although high density lipoprotein\u2010CE selective uptake has been shown to be linked to cell cholesterol homeostasis in nonhepatic cells, there is no available information on the effect of LDL\u2010CE selective uptake on hepatic cell cholesterol homeostasis. In order to define the role of the LDL\u2010CE selective uptake pathway in hepatic cell cholesterol homeostasis, we used a cellular model that expresses constitutively a LDLr antisense mRNA and that shows LDLr activity at 31% the normal level (HepG2\u2010all cells). The addition of a specific antibody anti\u2010LDLr (IgG\u2010C7) reduces LDL protein degradation (LDLr activity) to 7%. This cellular model therefore reflects, above all, LDL\u2010CE selective uptake activity when incubated with LDL. The inactivation of LDLr reduces LDL\u2013protein association by 78% and LDL\u2013CE association by only 43%. The LDL\u2010CE selective uptake was not reduced by the inactivation of LDLr. The activities of the various enzymes involved in cell cholesterol homeostasis were measured in normal and LDLr\u2010deficient cells during incubation in the absence or presence of LDL as a cholesterol source. Essentially, 3\u2010hydroxy\u20103\u2010methylglutaryl coenzyme A reductase and acyl coenzyme A:cholesterol acyltransferase (ACAT) activities responded to LDL in LDLr\u2010deficient cells as well as in normal HepG2 cells. Inhibition of lysosomal hydrolysis with chloroquine abolished the effect measured on ACAT activity in the presence of LDL, suggesting that CE of LDL, but not free cholesterol, maintains cell cholesterol homeostasis. Thus, in HepG2 cells, when LDLr function is virtually abolished, LDL\u2010CE selective uptake is coupled to cell cholesterol homeostasis.<\/jats:p>","DOI":"10.1046\/j.1432-1327.1999.00527.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:27:02Z","timestamp":1047403622000},"page":"402-409","source":"Crossref","is-referenced-by-count":10,"title":["Selective uptake of cholesteryl ester from low density lipoprotein is involved in HepG2 cell cholesterol homeostasis"],"prefix":"10.1111","volume":"263","author":[{"given":"Marie\u2010Claude","family":"Charest","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Rhainds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Falstrault","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony","family":"Matzouranis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Brissette","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/279679a0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"7306","DOI":"10.1016\/S0021-9258(19)42106-6","article-title":"Suppression of 3\u2010hydroxy\u20103methylglutaryl coenzyme A reductase activity and inhibition of growth in human fibroblasts by ketocholesterol.","volume":"249","author":"Brown M.S.","year":"1974","journal-title":"J. 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