{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T02:10:43Z","timestamp":1773627043177,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":664,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>Laccases are multicopper\u2010containing enzymes which catalyse the oxidation of phenolic and nonphenolic compounds with the concomitant reduction of molecular oxygen. In this study, a full\u2010length cDNA coding for laccase (<jats:italic>lac1<\/jats:italic>) from <jats:italic>Pycnoporus\u2003cinnabarinus<\/jats:italic> I\u2010937 was isolated and characterized. The corresponding open reading frame is 1557 nucleotides long and encodes a protein of 518 amino acids. The cDNA encodes a precursor protein containing a 21 amino\u2010acid signal sequence corresponding to a putative signal peptide. The deduced amino\u2010acid sequence of the encoded protein was similar to that of other laccase proteins, with the residues involved in copper coordination sharing the greatest extent of similarity. The cDNA encoding for laccase was placed under the control of the alcohol oxidase (Aox\u20031) promoter and expressed in the methylotropic yeast <jats:italic>Pichia\u2003pastoris<\/jats:italic>. The laccase leader peptide, as well as the <jats:italic>Saccharomyces\u2003cerevisiae<\/jats:italic>\u03b1\u2010factor signal peptide, efficiently directed the secretion into the culture medium of laccase in an active form. Moreover, the laccase activity was directly detected in plates. The identity of the recombinant product was further confirmed by protein immunoblotting. The expected molecular mass of the mature protein is 81\u2003kDa. However, the apparent molecular mass of the recombinant protein is 110\u2003k\u2003Da, thus suggesting that the protein expressed in <jats:italic>P.\u2003pastoris<\/jats:italic> may be hyperglycosylated.<\/jats:p>","DOI":"10.1046\/j.1432-1327.2000.01166.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:04:33Z","timestamp":1047405873000},"page":"1619-1625","source":"Crossref","is-referenced-by-count":98,"title":["Molecular cloning of the cDNA encoding laccase from <i>Pycnoporus cinnabarinus<\/i> I\u2010937 and expression in <i>Pichia\u2003pastoris<\/i>"],"prefix":"10.1111","volume":"267","author":[{"given":"Ludovic","family":"Otterbein","sequence":"first","affiliation":[]},{"given":"Eric","family":"Record","sequence":"additional","affiliation":[]},{"given":"Sonia","family":"Longhi","sequence":"additional","affiliation":[]},{"given":"Marcel","family":"Asther","sequence":"additional","affiliation":[]},{"given":"Serge","family":"Moukha","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"765","article-title":"Polyphenol oxidases in plants.","volume":"33","author":"Mayer A.M.","year":"1979","journal-title":"Phytochemistry"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80298-W"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1994.6020213.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1128\/aem.62.4.1151-1158.1996","article-title":"The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase.","volume":"62","author":"Eggert C.","year":"1996","journal-title":"Appl. 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