{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:04:12Z","timestamp":1761523452451},"reference-count":53,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":542,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p>Two clones were isolated in a three\u2010hybrid screen of a rat fetal brain P5 cDNA library with an intronic splicing enhancer of the amyloid precursor protein (APP) gene as RNA bait. These clones represent the rat homologues of the previously described genes CUG\u2010binding protein (CUG\u2010BP) and Siah\u2010binding protein (Siah\u2010BP). Both interact in a sequence\u2010specific manner with the RNA bait used for library screening as well as with the CUG repeat. In contrast, no interactions were observed in the three\u2010hybrid assay with other baits tested. In two\u2010hybrid assays, Siah\u2010BP interacts with U2AF65 as well as with itself. EWS, an RGG\u2010type RNA\u2010binding protein associated with Ewing sarcoma, was identified as an interacting partner for the CUG\u2010BP homologue in a two\u2010hybrid assay for protein\u2013protein interactions performed with various factors involved in RNA metabolism. Splicing assays performed by RT\u2010PCR from cells cotransfected with certain cDNAs and an APP minigene, used as a reporter, indicate exclusion of exon\u20038 if the CUG\u2010BP homologue is present. We conclude that clone AF169013 and its counterpart in human CUG\u2010BP could be the<jats:italic>trans<\/jats:italic>\u2010acting factors that interact with the splicing enhancer downstream of exon\u20038, and in this way influence alternative splicing of the APP minigene.<\/jats:p>","DOI":"10.1046\/j.1432-1327.2000.01431.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:47:33Z","timestamp":1047404853000},"page":"4002-4010","source":"Crossref","is-referenced-by-count":32,"title":["A<i>trans<\/i>\u2010acting factor, isolated by the three\u2010hybrid system, that influences alternative splicing of the amyloid precursor protein minigene"],"prefix":"10.1111","volume":"267","author":[{"given":"Andrej","family":"Poleev","sequence":"first","affiliation":[]},{"given":"Annette","family":"Hartmann","sequence":"additional","affiliation":[]},{"given":"Stefan","family":"Stamm","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0968-0004(00)01549-8"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80007-5"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.5.786"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90298-5"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.3.269"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)70787-2"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90291-J"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.21.2659"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.8.1023"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80900-9"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00216-d"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0169-328x(97)00045-4"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00150-3"},{"key":"e_1_2_7_15_2","first-page":"1275","article-title":"A unique intronic splicing enhancer controls the inclusion of the agrin Y exon","volume":"3","author":"Wei N.","year":"1997","journal-title":"RNA"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0169-328x(98)00313-1"},{"key":"e_1_2_7_17_2","doi-asserted-by":"crossref","DOI":"10.1093\/oso\/9780199636518.001.0001","volume-title":"RNA\u2013protein interactions. 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