{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:15:50Z","timestamp":1760296550509},"reference-count":43,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":511,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>We report the intracellular inhibition of blood group A<jats:italic>N<\/jats:italic>\u2010acetylgalactosaminyltransferase in the human colorectal carcinoma cell line HT29 by 3\u2010amino\u20103\u2010deoxy\u2010[Fuc\u03b1(1\u20132)]Gal\u03b2\u2010O(CH<jats:sub>2<\/jats:sub>)<jats:sub>7<\/jats:sub>CH<jats:sub>3<\/jats:sub>. Inhibition was demonstrated with a novel capillary electrophoresis assay that monitored decreased intracellular conversion of fluorescently labelled Fuc\u03b1(1\u20132)Gal\u2010R acceptor to the corresponding A epitope, GalNAc\u03b1(1\u20133)[Fuc\u03b1(1\u20132)]Gal\u03b2\u2010R. Growth of HT29 cells with either the amino\u2010inhibitor or a competitive substrate, Fuc\u03b1(1\u20132)Gal\u03b2\u2010O(CH<jats:sub>2<\/jats:sub>)<jats:sub>7<\/jats:sub>CH<jats:sub>3<\/jats:sub>, also resulted in decreased expression of blood group A determinants on cell\u2010associated glycoproteins, as detected by immunoprecipitation analysis using A\u2010specific monoclonal antibodies. Furthermore, exposure of these cells to the amino\u2010inhibitor or competitive substrate resulted in significant reduction of cell\u2010surface expression of blood group A determinants. As integrin \u03b13\u03b21, a cell\u2010surface receptor mediating cell\u2013cell and cell\u2013extracellular matrix interactions, was shown previously to be a major carrier of blood group A determinants on HT29 cells, the studies described herein highlight the potential usefulness of these compounds for elucidating the role of blood group A determinants in biological phenomena.<\/jats:p>","DOI":"10.1046\/j.1432-1327.2000.01544.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T17:50:33Z","timestamp":1047405033000},"page":"4840-4849","source":"Crossref","is-referenced-by-count":29,"title":["Intracellular inhibition of blood group A glycosyltransferase"],"prefix":"10.1111","volume":"267","author":[{"given":"Suzanne","family":"Lafert\u00e9","sequence":"first","affiliation":[]},{"given":"Nora W. 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