{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:05:47Z","timestamp":1778180747179,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":449,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Eur J of Neuroscience"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatocyte growth factor\u2010scatter factor (HGF) is expressed in different parts of the nervous system, and has been shown to exhibit neurotrophic activity. Here we show that c\u2010Met, the receptor for HGF, is expressed in developing rat hippocampus, with the highest levels during the first postnatal weeks. To study the function of HGF, hippocampal neurons were prepared from embryonic rats and treated with different HGF concentrations. In these cultures, HGF increased the number of neurons expressing the 28\u2010kDa calcium\u2010binding protein (calbindin D) in a dose\u2010dependent manner. The effect of HGF was larger than that observed with either brain\u2010derived neurotrophic factor (BDNF) or neurotrophin\u20103 (NT\u20103), and cotreatment of the cultures with HGF and the neurotrophins was additive with respect to calbindin D neurons. Besides affecting the number of neurons, HGF significantly increased the degree of sprouting of calbindin D\u2010positive neurons, suggesting an influence on neuronal maturation. BDNF and NT\u20103 stimulated neurite outgrowth of calbindin D neurons to a much smaller degree. In contrast to calbindin D neurons, HGF did not significantly increase the number of neurons immunoreactive with the neurotransmitter \u03b3\u2010aminobutyric acid (GABA) in the hippocampal cultures. Immunohistochemical studies showed that c\u2010Met\u2010, calbindin D\u2010 and HGF\u2010immunoreactive cells are all present in the dentate gyrus and partly colocalize within neurons. These results show that HGF acts on calbindin D\u2010containing hippocampal neurons and increases their neurite outgrowth, suggesting that HGF plays an important role for the maturation and function of these neurons in the hippocampus.<\/jats:p>","DOI":"10.1046\/j.1460-9568.2000.00260.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:27:57Z","timestamp":1046960877000},"page":"3453-3461","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Expression of c\u2010Met in developing rat hippocampus: evidence for HGF as a neurotrophic factor for calbindin D\u2010expressing neurons"],"prefix":"10.1111","volume":"12","author":[{"given":"Laura","family":"Korhonen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrika","family":"Sj\u00f6holm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Takei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Kern","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Schirmacher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eero","family":"Castr\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Lindholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(97)00108-9"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/376768a0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1846706"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.3.629"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90091-H"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90028-C"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1242\/dev.116.2.387","article-title":"The presence of hepatocyte growth factor in the developing rat","volume":"116","author":"De Frances M.","year":"1992","journal-title":"Development"},{"key":"e_1_2_6_10_1","first-page":"219","article-title":"Selective expression of the met\/HGF receptor in human central nervous system microglia","volume":"8","author":"Di Renzo M.F.","year":"1993","journal-title":"Oncogene"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80247-0"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"5714","DOI":"10.1523\/JNEUROSCI.18-15-05714.1998","article-title":"Nerve growth factor induced process formation in meningeal cells: Implications for scar formation in the injured CNS","volume":"18","author":"Fris\u00e9n J.","year":"1998","journal-title":"J. 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