{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:13:02Z","timestamp":1769641982105,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2008,7,7]],"date-time":"2008-07-07T00:00:00Z","timestamp":1215388800000},"content-version":"vor","delay-in-days":2532,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p>\n                    Diffusion of octane from oily droplets in different microscale settings was measured using\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    expressing the stable green fluorescent protein (GFP) from the\n                    <jats:italic>alkB<\/jats:italic>\n                    promoter of\n                    <jats:italic>Pseudomonas oleovorans<\/jats:italic>\n                    . GFP fluorescence intensities were determined quantitatively at the single\u2010cell level after 1.0 or 2.5\u2003h incubation and compared with different calibration series using known concentrations of octane. By immobilizing the\n                    <jats:italic>E. coli<\/jats:italic>\n                    sensor cells on the bottom glass plate of a microscope flow chamber, it was possible to monitor the diffusion process for octane in aqueous solution as a function of time and distance from non\u2010aqueous phase droplets of octane alone or oily octane mixtures. When a gas phase was included in the flow chambers, octane transport could be demonstrated from the oily mixtures to the cells through both gas and liquid phase. Assays of non\u2010immobilized sensor cells in microdroplets in the presence or absence of soil particles incubated with octane through the vapour phase revealed a slight reduction in the total amount of induced\n                    <jats:italic>E. coli<\/jats:italic>\n                    cells\u00a0in the presence of soil. Our results indicate the power of using GFP\u2010marked single\u2010cell biosensors in determining microscale bioavailability of organic pollutants.\n                  <\/jats:p>","DOI":"10.1046\/j.1462-2920.2001.00218.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T14:21:47Z","timestamp":1047392507000},"page":"512-524","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Measuring mass transfer processes of octane with the help of an\n                    <i>alkS<\/i>\n                    <i>alkB::gfp<\/i>\n                    \u2010tagged\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1111","volume":"3","author":[{"given":"Marco C. 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