{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:25:35Z","timestamp":1768350335930,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,12,9]],"date-time":"2002-12-09T00:00:00Z","timestamp":1039392000000},"content-version":"vor","delay-in-days":38,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["enviromicro-journals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Environmental Microbiology"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Sulphide mineral mining together with improperly contained sulphur\u2010rich coal represents a significant environmental problem caused by leaching of toxic material. The Savannah River Site's D\u2010area harbours a 22\u2010year\u2010old exposed reject coal pile (RCP) from which acidic, metal rich, saline runoff has impacted an adjacent forested wetland. In order to assess the bacterial community composition of this region, composite sediment samples were collected at three points along a contamination gradient (high, middle and no contamination) and processed for generation of bacterial and archaeal 16S rDNA clone libraries. Little sequence overlap occurred between the contaminated (RCP samples) and unimpacted sites, indicating that the majority of 16S rDNAs retrieved from the former represent organisms selected by the acidic runoff. Archaeal diversity within the RCP samples consisted mainly of sequences related to the genus\n                    <jats:italic>Thermoplasma<\/jats:italic>\n                    and to sequences of a novel type. Bacterial RCP libraries contained 16S rRNA genes related to isolates (\n                    <jats:italic>Acidiphilium<\/jats:italic>\n                    sp.,\n                    <jats:italic>Acidobacterium capsulatum, Ferromicrobium acidophilium<\/jats:italic>\n                    and\n                    <jats:italic>Leptospirillum ferrooxidans<\/jats:italic>\n                    ) and environmental clones previously retrieved from acidic habitats, including ones phylogenetically associated with organisms capable of sulphur and iron metabolism. These libraries also exhibited particularly novel 16S rDNA types not retrieved from other acid mine drainage habitats, indicating that significant diversity remains to be detected in acid mine drainage\u2010type systems.\n                  <\/jats:p>","DOI":"10.1046\/j.1462-2920.2002.00337.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T14:22:13Z","timestamp":1047392533000},"page":"764-769","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":97,"title":["Recovery of novel bacterial diversity from a forested wetland impacted by reject coal"],"prefix":"10.1111","volume":"4","author":[{"given":"Jennifer E.","family":"Brofft","sequence":"first","affiliation":[]},{"given":"J. Vaun","family":"McArthur","sequence":"additional","affiliation":[]},{"given":"Lawrence J.","family":"Shimkets","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,12,9]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007920070018"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002480000063"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.9.3842-3849.2000"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.11.4962-4971.2000"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00170748"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01257118"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.2134\/jeq1981.00472425001000020001x"},{"key":"e_1_2_4_9_1","doi-asserted-by":"crossref","first-page":"3869","DOI":"10.1128\/AEM.64.10.3869-3877.1998","article-title":"Microbial diversity in a hydrocarbon\u2010 and clorinated\u2010 solvent\u2010contaminated aquifer undergoing intrinsic bioremediation","volume":"64","author":"Dojka M.A.","year":"1998","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.4.1617-1621.2000"},{"key":"e_1_2_4_11_1","first-page":"155","article-title":"Geomicrobiology of pyrtie (FeS2) dissolution: a case study at Iron Mountain, California","volume":"16","author":"Edwards K.J.","year":"1998","journal-title":"Geomicrobiol J"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-1869-3_15"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-4-1539"},{"key":"e_1_2_4_14_1","volume-title":"Analytic Rarefaction 1.3","author":"Holland S.M.","year":"2001"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.1998.tb00547.x"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-2-511"},{"key":"e_1_2_4_17_1","first-page":"115","volume-title":"Nucleic Acid Sequencing Techniques in Bacterial Systematics","author":"Lane D.J.","year":"1991"},{"key":"e_1_2_4_18_1","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1128\/aem.62.7.2501-2507.1996","article-title":"A computer\u2010stimulated restriction fragment length polymorphism analysis of bacterial small\u2010subunit rRNA genes: Efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature","volume":"62","author":"Moyer C.L.","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.4.1874-1884.2001"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00446317"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.4.1323-1328.1996"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3724-3730.1998"},{"key":"e_1_2_4_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01574689"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.9.4374-4376.2001"},{"key":"e_1_2_4_25_1","doi-asserted-by":"crossref","first-page":"4522","DOI":"10.1128\/AEM.64.11.4522-4529.1998","article-title":"Kinetic bias in estimates of coastal picoplankton community structure by measurements of small\u2010subunit rRNA gene PCR amplicon length heterogeneity","volume":"64","author":"Suzuki M.","year":"1998","journal-title":"Appl Environ Microbiol"}],"container-title":["Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1462-2920.2002.00337.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1462-2920.2002.00337.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T04:12:23Z","timestamp":1761883943000},"score":1,"resource":{"primary":{"URL":"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/10.1046\/j.1462-2920.2002.00337.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":24,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1046\/j.1462-2920.2002.00337.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1462-2920.2002.00337.x","archive":["Portico"],"relation":{},"ISSN":["1462-2912","1462-2920"],"issn-type":[{"value":"1462-2912","type":"print"},{"value":"1462-2920","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]},"assertion":[{"value":"2002-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}