{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:24:37Z","timestamp":1773660277584,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,7,7]],"date-time":"2008-07-07T00:00:00Z","timestamp":1215388800000},"content-version":"vor","delay-in-days":2713,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>The phosphorylation state of \u03b1B\u2010crystallin accumulated in the brains of two patients with Alexander's disease (one infantile and one juvenile type) was determined by means of SDS\u2010PAGE or isoelectric focusing of soluble and insoluble fractions of brain extracts and subsequent western blot analysis with specific antibodies against \u03b1B\u2010crystallin and each of three phosphorylated serine residues. The level of mammalian small heat shock protein of 25\u201328\u2003kDa (Hsp27) in the same fraction was also estimated by western blot analysis. The majority of \u03b1B\u2010crystallin was detected in the insoluble fraction of brain homogenates and phosphorylation was preferentially observed at Ser\u201059 in both cases. A significant level of phosphorylation at Ser\u201045 but not Ser\u201019 was also detected. Hsp27 was found at considerable levels in the insoluble fractions. \u03b1B\u2010crystallin and phosphorylated forms were detected in the cerebrospinal fluid of patient with the juvenile type. \u03b1B\u2010crystallin and phosphorylated forms were also detectable at considerable levels in the insoluble fraction of brain homogenates from patients with Alzheimer's disease and aged controls. The phosphorylation site was mostly at Ser\u201059 in all cases. Immunohistochemically, \u03b1B\u2010crystallin was stained in Rosenthal fibers in brains of patients with Alexander's disease and their peripheral portions were immunostained with antibodies recognizing phosphorylated Ser\u201059. These results indicate that the major phosphorylation site in \u03b1B\u2010crystallin in brains of patients with Alexander's disease or Alzheimer's disease as well as in aged controls is Ser\u201059.<\/jats:p>","DOI":"10.1046\/j.1471-4159.2001.00038.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:51:05Z","timestamp":1047451865000},"page":"730-736","source":"Crossref","is-referenced-by-count":49,"title":["Ser\u201059 is the major phosphorylation site in \u03b1B\u2010crystallin accumulated in the brains of patients with Alexander's disease"],"prefix":"10.1111","volume":"76","author":[{"given":"Kanefusa","family":"Kato","sequence":"first","affiliation":[]},{"given":"Yutaka","family":"Inaguma","sequence":"additional","affiliation":[]},{"given":"Hidenori","family":"Ito","sequence":"additional","affiliation":[]},{"given":"Kayo","family":"Iida","sequence":"additional","affiliation":[]},{"given":"Ikuko","family":"Iwamoto","sequence":"additional","affiliation":[]},{"given":"Keiko","family":"Kamei","sequence":"additional","affiliation":[]},{"given":"Nobuhiko","family":"Ochi","sequence":"additional","affiliation":[]},{"given":"Hideomi","family":"Ohta","sequence":"additional","affiliation":[]},{"given":"Masao","family":"Kishikawa","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,7,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050862"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.54.5.1033"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90787-9"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00163229"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-3940(97)00521-1"},{"key":"e_1_2_7_7_1","first-page":"533","volume-title":"Molecular Chaperones in\u00a0the Life Cycle of Proteins","author":"Ehrnsperger M.","year":"1998"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"2093","DOI":"10.1046\/j.1471-4159.1999.02093.x","article-title":"Alpha\u2010synuclein immunoisolation of glial inclusions from multiple system atrophy brain tissue reveals multiprotein components","volume":"73","author":"Gai W. P.","year":"1999","journal-title":"J. Neurochem."},{"key":"e_1_2_7_9_1","first-page":"1743","article-title":"Overexpression and abnormal modification of the stress proteins alpha B\u2010crystallin and HSP27 in Alexander disease","volume":"143","author":"Head M. W.","year":"1993","journal-title":"Am. J. Pathol."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.47.29934"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90173-6"},{"key":"e_1_2_7_12_1","first-page":"487","article-title":"Alpha B\u2010crystallin and 27\u2010kd heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers","volume":"143","author":"Iwaki T.","year":"1993","journal-title":"Am. J. Pathol."},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0141-8130(98)00013-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(91)90062-L"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42574-4"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.43.28346"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)81359-G"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/375798a0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91922-Y"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(93)90135-L"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/10.7.943"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0304-3940(97)00336-4"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0387-7604(00)00117-0"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1471-4159.2001.00038.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1471-4159.2001.00038.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T17:56:22Z","timestamp":1704304582000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1471-4159.2001.00038.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1046\/j.1471-4159.2001.00038.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1471-4159.2001.00038.x","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}