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However, the molecular mechanisms responsible for recruiting\/sorting the \u03b1 chains to the signaling chains at the cell surface are not clear. Here we show, in four cell lines of human adult T\u2003cell lymphoma\/leukemia origin, that the three IL\u20102R subunits are compartmented together with HLA glycoproteins and CD48 molecules in the plasma membrane, by means of fluorescence resonance energy transfer (FRET), confocal microscopy and immuno\u2010biochemical techniques. In addition to the \u03b2 and \u03b3<jats:sub>c<\/jats:sub> chains constitutively expressed in detergent\u2010resistant membrane fractions (DRMs) of T\u2003cells, IL\u20102R\u03b1 (CD25) was also found in DRMs, independently of its ligand\u2010occupation. Association of CD25 with rafts was also confirmed by its colocalization with GM\u20101 ganglioside. Depletion of membrane cholesterol using methyl\u2010\u03b2\u2010cyclodextrin substantially reduced co\u2010clustering of CD25 with CD48 and HLA\u2010DR, as well as the IL\u20102 stimulated tyrosine\u2010phosphorylation of STATs (signal transducer and activator of transcription). These data indicate a GPI\u2010microdomain (raft)\u2010assisted recruitment of CD25 to the vicinity of the signaling \u03b2 and \u03b3<jats:sub>c<\/jats:sub> chains. Rafts may promote rapid formation of a high affinity IL\u20102R complex, even at low levels of IL\u20102 stimulus, and may also form a platform for the regulation of IL\u20102 induced signals by GPI\u2010proteins (e.g. CD48). Based on these data, the integrity of these GPI\u2010microdomains seems critical in signal transduction through the IL\u20102R complex.<\/jats:p>","DOI":"10.1046\/j.0014-2956.2002.02759.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:39:06Z","timestamp":1047407946000},"page":"1199-1208","source":"Crossref","is-referenced-by-count":74,"title":["GPI\u2010microdomains (membrane rafts) and signaling of the multi\u2010chain interleukin\u20102 receptor in human lymphoma\/leukemia T\u2003cell lines"],"prefix":"10.1111","volume":"269","author":[{"given":"J\u00e1nos","family":"Matk\u00f3","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Bodn\u00e1r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy","family":"Vereb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e1szl\u00f3","family":"Bene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy","family":"V\u00e1mosi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gergely","family":"Szentesi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1nos","family":"Sz\u00f6ll\u00f6si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Rezs\u00f5","family":"G\u00e1sp\u00e1r","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"Horejsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Waldmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e1ndor","family":"Damjanovich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,2,20]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)49895-X"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60386-7"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/369330a0"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.293"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80246-0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00208-9"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.129.1.55"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(94)90130-9"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/11.suppl_1.S101"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.23.9026"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.24.13134"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.11.6013"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80577-4"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(99)01489-9"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(99)80017-9"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0033583500004637"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050216"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(84)84240-X"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(97)78023-6"},{"key":"e_1_2_7_21_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/B978-0-12-417760-4.50012-4","volume-title":"Cell Structure and Functionby Microspectrofluorimetry","author":"Jovin T.M.","year":"1989"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/29563"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19981001)33:2<225::AID-CYTO18>3.3.CO;2-6"},{"key":"e_1_2_7_24_2","doi-asserted-by":"crossref","first-page":"5430","DOI":"10.4049\/jimmunol.161.10.5430","article-title":"IL\u20102R alpha on one cell can present IL\u20102 to IL\u20102R beta\/gamma (c) on another cell to augment IL\u20102 signaling","volume":"161","author":"Eicher D.M.","year":"1998","journal-title":"J. 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