{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:27:54Z","timestamp":1754396874018},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,11,7]],"date-time":"2003-11-07T00:00:00Z","timestamp":1068163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold><jats:bold> Objectives:<\/jats:bold> Beta\u2010thalassaemia minor (BTM) alone does not lead to iron overload, however, some gene modifiers and acquired causes are reported. When it is inherited together with a mutation in the HFE (HLA\u2010H) gene associated with hereditary haemochromatosis, iron overload may ensue. To analyse the relationship between iron status and HFE mutations in Iranian BTM patients, we compared the frequency of the C282Y and H63D HFE mutations and ferritin level in a group of BTM patients from the National Thalassaemia Transfusion and Care Centre with that of healthy individuals.<\/jats:p><jats:p><jats:bold>Patients and methods:<\/jats:bold> Ninety\u2010three (56 females) documented BTM cases and 104 (54 females) controls were enrolled in the study. Serum ferritin level was measured in all subjects by immuno\u2010radiometric assay and HFE genotypes were determined using restriction fragment length polymorphism analysis of PCR\u2010amplified HFE gene fragment.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Eighteen (19.4%) BTM patients vs. 12 (11.5%) controls were H63D heterozygotes, while there were three (3.2%) cases and three (2.9%) controls with H63D homozygosity. All three C282Y mutations were found in BMT patients with one of them being a compound heterozygote. A significant difference was observed in the total number of HFE mutations in favour of BTM patients over the controls (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05, OR\u2003=\u20032.064). The H63D and C282Y allele frequencies were 12.9 and 1.61 in patients and 8.65 and 0 in controls, respectively. The mean ferritin level in cases with HFE mutations showed no significant difference from that of the patients without mutations (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our results suggest that HFE mutations C282Y and H63D are more frequent in Iranian BTM patients than in the normal population, causing no significant changes in serum ferritin level.<\/jats:p>","DOI":"10.1046\/j.0902-4441.2003.00159.x","type":"journal-article","created":{"date-parts":[[2003,11,7]],"date-time":"2003-11-07T20:38:38Z","timestamp":1068237518000},"page":"408-411","source":"Crossref","is-referenced-by-count":16,"title":["Frequency of HFE gene mutations in Iranian beta\u2010thalassaemia minor patients"],"prefix":"10.1111","volume":"71","author":[{"given":"Maryam","family":"Jazayeri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valery","family":"Bakayev","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peyman","family":"Adibi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farhad Haghighi","family":"Rad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid","family":"Zakeri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ebrahim","family":"Kalantar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Reza","family":"Zali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,11,7]]},"reference":[{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)08246-X"},{"key":"e_1_2_12_3_2","first-page":"134","article-title":"Regional mapping of the gene frequency of b\u2010thalassemia in Fars province, Iran during 1997\u20131998","volume":"5","author":"Karimi M","year":"2000","journal-title":"Irn J Med Sci"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002770050600"},{"key":"e_1_2_12_5_2","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1093\/ajcp\/70.6.861","article-title":"Population screening for beta\u2010thalassemia minor. 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