{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:31:58Z","timestamp":1778859118659,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,16]],"date-time":"2003-04-16T00:00:00Z","timestamp":1050451200000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> Pemphigus vulgaris (PV) is an autoimmune blistering skin disease of humans and companion animals. In human patients, PV is associated with the production of IgG autoantibodies specific for keratinocyte desmosomal glycoproteins of the cadherin family. The purpose of this study was to determine whether antikeratinocyte IgG autoantibodies were present in the skin and serum of dogs with PV, and also to identify the canine PV autoantigen(s) targeted by circulating autoantibodies. Eleven dogs were selected because of the microscopic demonstration of suprabasal epithelial acantholysis. Direct immunofluorescence revealed the presence of IgG autoantibodies bound to the membrane of keratinocytes in skin biopsy specimens of 8\/9 dogs (89%). Using indirect immunofluorescence, serum\u2010circulating IgG autoantibodies were found in 10\/11 (91%) and 5\/11 (45%) dogs, using normal canine gingiva and cultured canine oral keratinocytes, respectively. By immunoblotting using cultured canine oral keratinocyte protein lysates, IgG autoantibodies from 7\/9 (78%) tested dogs recognized a 130\u2010kDa antigen that comigrated with that identified by rabbit polyclonal antibodies raised against desmoglein\u20103. This 130\u2003kDa antigen was confirmed to represent the canine equivalent of human desmoglein\u20103 by immunoprecipitation\u2010immunoblotting. The results of these studies provide evidence that the canine desmoglein\u20103 homologue is a major autoantigen in dogs with PV. These observations further establish spontaneous canine PV as a natural model for research on pathogenesis, etiology and novel therapeutic approaches for this disease of humans.<\/jats:p>","DOI":"10.1046\/j.0906-6705.2002.072.x","type":"journal-article","created":{"date-parts":[[2004,12,22]],"date-time":"2004-12-22T22:07:46Z","timestamp":1103753266000},"page":"198-203","source":"Crossref","is-referenced-by-count":25,"title":["Desmoglein\u20103 is a target autoantigen in spontaneous canine pemphigus vulgaris"],"prefix":"10.1111","volume":"12","author":[{"given":"Thierry","family":"Olivry","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohaila","family":"Joubeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley M.","family":"Dunston","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Nishiyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza F.","family":"Ghohestani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,4,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-6757(00)90124-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)70183-0"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003174200"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64651-2"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11828"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2001.01307.x"},{"key":"e_1_2_6_8_2","first-page":"575","article-title":"A mucocutaneous disease in the dog resembling pemphigus vulgaris in Man","volume":"166","author":"Stannard A A","year":"1975","journal-title":"J Am Vet Med Assn"},{"key":"e_1_2_6_9_2","first-page":"585","article-title":"A disease in dogs resembling human pemphigus vulgaris: case reports","volume":"166","author":"Hurvitz A I","year":"1975","journal-title":"J Am Vet Med Assn"},{"key":"e_1_2_6_10_2","first-page":"164","article-title":"Pemphigus vulgaris in the dog: a case report","volume":"13","author":"Hoskins J D","year":"1977","journal-title":"J Am Anim Hosp Assn"},{"key":"e_1_2_6_11_2","first-page":"317","article-title":"Pemphigus vulgaris in a border collie","volume":"19","author":"Parker W M","year":"1978","journal-title":"Can Vet J"},{"key":"e_1_2_6_12_2","first-page":"249","article-title":"[Pemphigus vulgaris and pemphigus foliaceus in the dog \u2013 report of 9 cases]","volume":"126","author":"Suter M","year":"1984","journal-title":"Schweiz Arch Tierheilkd"},{"key":"e_1_2_6_13_2","first-page":"168","article-title":"Canine pemphigus vulgaris treated with gold salt therapy","volume":"25","author":"Olynyk G P","year":"1984","journal-title":"Can Vet J"},{"key":"e_1_2_6_14_2","first-page":"217","article-title":"[Pemphigus vulgaris in a dog \u2013 and your diagnosis]","volume":"72","author":"Brabenetz J","year":"1985","journal-title":"Wien Tierarztl Monatsschr"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.1992.tb00149.x"},{"key":"e_1_2_6_16_2","first-page":"75","article-title":"[A case of pemphigus vulgaris in a German shorthaired pointer]","volume":"29","author":"Bensignor E","year":"1998","journal-title":"Point Vet"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.37-5-496"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.148.14.450"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04311.x"},{"key":"e_1_2_6_20_2","first-page":"401","article-title":"Pemphigus vulgaris without mucosal or mucocutaneous involvement in two dogs","volume":"18","author":"Scott D W","year":"1982","journal-title":"J Am Anim Hosp Assn"},{"key":"e_1_2_6_21_2","first-page":"424","article-title":"Immune\u2010mediated dermatoses in domestic animals. 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