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Both PLC activation and Ca<jats:sup>2+<\/jats:sup> signalling were found to be mediated exclusively by the mGluR1 subtype, although both group\u2003I mGluRs, mGluR1\u03b1 and mGluR5, could be detected in cell extracts. Exposure of cells to medium devoid of Ca<jats:sup>2+<\/jats:sup> for various times before agonist stimulation reduced the PLC response, which was quickly recovered following the re\u2010exposure of cells to Ca<jats:sup>2+<\/jats:sup>\u2010containing medium. The extent of the glutamate response correlated well with changes in the cytosolic Ca<jats:sup>2+<\/jats:sup> concentration. On the other hand, loading of the intracellular Ca<jats:sup>2+<\/jats:sup> stores by a transient depolarization followed by washing in nondepolarizing buffer, allowed glutamate to release stored Ca<jats:sup>2+<\/jats:sup> in the majority of cells and enhanced glutamate activation of PLC. Under such conditions, the absence of extracellular Ca<jats:sup>2+<\/jats:sup> during stimulation and the chelation of cytosolic Ca<jats:sup>2+<\/jats:sup> with BAPTA\/AM inhibited both glutamate\u2010elicited Ca<jats:sup>2+<\/jats:sup> response and PLC activation. Overall, these results indicate that the mGluR\u2010mediated activation of PLC depends on the presence of extracellular Ca<jats:sup>2+<\/jats:sup> and can be modulated by moderate changes of cytosolic Ca<jats:sup>2+<\/jats:sup>. Furthermore, ryanodine reduced PLC stimulation by glutamate in predepolarized cells but not in control cells, suggesting that ryanodine receptors could play a role in the potentiation of the mGluR\u2010mediated activation of PLC by Ca<jats:sup>2+<\/jats:sup> release in predepolarized cells.<\/jats:p>","DOI":"10.1046\/j.0953-816x.2000.01384.x","type":"journal-article","created":{"date-parts":[[2003,3,6]],"date-time":"2003-03-06T14:36:58Z","timestamp":1046961418000},"page":"248-256","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Characterization of the metabotropic glutamate receptors mediating phospholipase\u2003C activation and calcium release in cerebellar granule cells: calcium\u2010dependence of the phospholipase\u2003C response"],"prefix":"10.1111","volume":"13","author":[{"given":"Roser","family":"Masgrau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan\u2010Marc","family":"Servitja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth W.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Pardo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabet","family":"Sarri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan R.","family":"Nahorski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Picatoste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1993.tb03185.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(95)00079-l"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/366575a0"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1997.tb01348.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/382719a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.37.1.205"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.1998.00230.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst019154s"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028-3908(97)00001-4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-2236(99)01492-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0922-4106(93)90106-J"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028-3908(99)00082-9"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028-3908(98)00132-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1998.70041772.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(92)90064-Y"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/386239a0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80684-4"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5357.1722"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702464"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.740818.x"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0028-3908(99)00082-9"},{"key":"e_1_2_6_24_1","first-page":"721","article-title":"Pharmacological characterization of 1\u2010aminoindan\u20101,5\u2010dicarboxylic acid, a potent mGluR1 antagonist","volume":"281","author":"Moroni F.","year":"1997","journal-title":"J. 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