{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T23:22:08Z","timestamp":1774567328469,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,11,21]],"date-time":"2002-11-21T00:00:00Z","timestamp":1037836800000},"content-version":"vor","delay-in-days":234,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\u2003<\/jats:bold> Correcting Th1\/Th2 imbalance with administration of IL\u201012 before and during antigen challenge holds therapeutic promise in asthma. However, the effects of IL\u201012 on the established asthmatic responses have not fully been examined.<\/jats:p><jats:p><jats:bold>Objective\u2003<\/jats:bold> We investigated whether IL\u201012 administered after antigen challenge could diminish airway hyper\u2010reactivity (AHR) and eosinophilia in mice actively sensitized to ovalbumin. We also have investigated the ability of administered IL\u201012 to induce IL\u201018 receptor (IL\u201018R) expression that may lead possible synergic action of IL\u201012 with endogenous IL\u201018.<\/jats:p><jats:p><jats:bold>Methods\u2003<\/jats:bold> C57BL\/6 mice immunized to ovalbumin (OVA) by intraperitoneal (i.p.) injection, were challenged three times with an aerosol of OVA every second day for 8\u2003days. Recombinant IL\u201012 (500\u2003ng) was intravenously administered on a single occasion 1\u2003h after the final challenge of mice. Mice were analysed for effects of IL\u201012 on AHR, inflammatory cell infiltration and cytokine levels in lung tissue as well as serum immunoglobulin (Ig) E levels. Immunohistochemistry for IL\u201018R was performed using rat monoclonal antibody specific for murine IL\u201018R\u03b1 (IL\u20101 receptor related protein; IL\u20101Rrp).<\/jats:p><jats:p><jats:bold>Results\u2003<\/jats:bold> An intravenous IL\u201012 administration diminished AHR, pulmonary eosinophilia and T lymphocyte infiltration, serum IgE, IL\u20104 and IL\u201013 in lung tissue. Expression of IL\u201018R was induced in the mononuclear cells in the lung of mice exposed to OVA. IL\u201012 administration enhanced the IL\u201018R expression compared with the control.<\/jats:p><jats:p><jats:bold>Conclusion\u2003<\/jats:bold> These data indicate that IL\u201012 can attenuate established antigen\u2010induced AHR and inflammation. In this mechanism it would be interpreted as follows: IL\u201012 administration in OVA\u2010challenged mice decreased IL\u20104 production and IgE production thereafter through direct effect on inhibiting the activation of established Th2 cells response and also combined effect with up\u2010regulation of IL\u201018R expression by inflammatory cells in the lung.<\/jats:p>","DOI":"10.1046\/j.0954-7894.2002.01346.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:51:33Z","timestamp":1047376293000},"page":"641-649","source":"Crossref","is-referenced-by-count":20,"title":["Effects of post\u2010inhalation treatment with interleukin\u201012 on airway hyper\u2010reactivity, eosinophilia and interleukin\u201018 receptor expression in a mouse model of 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