{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:38:29Z","timestamp":1773308309019,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,11,23]],"date-time":"2002-11-23T00:00:00Z","timestamp":1038009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Obstet and Gynaecol"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objective:<\/jats:bold> Our purpose was to assess the cerebral blood flow distribution and resulting grade of hypoxic\u2013ischemic brain damage in newborn rats.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Seven\u2010day\u2010old Wistar rats (<jats:italic>n<\/jats:italic>\u2003=\u200375) underwent left common carotid artery ligation followed by 2\u2003hours hypoxia (8% oxygen in nitrogen) at 33\u00b0C. The control animals were exposed to hypoxia without ligation (<jats:italic>n<\/jats:italic>\u2003=\u20038). Colored microspheres of 15\u2003\u00b5m in diameter were administered into the left cardiac ventricle percutaneously at the end of hypoxia. They were killed 24\u2003hours after induced injury. Brain sections 2\u2003mm in thickness were removed for microtubule\u2010associated protein\u20032 (MAP\u20102) staining, and remaining parts were separated into left and right hemispheres for counting the microspheres. The blood flow distribution to the ligated side was expressed as the difference from the non\u2010ligated control side. Severity of MAP\u20102 disappearance was ranked as normal, mild or severe.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In the control rats, there was no loss of MAP\u20102 staining. The blood flow equally distributed into both cerebral hemispheres. The cerebral blood flow distribution on the side of carotid artery ligation decreased by 44.7\u2003\u00b1\u200321.9% in the mildly damaged group and 65.8\u2003\u00b1\u200316.8% in the severely damaged group.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The greater the percentage difference of blood flow distribution from the non\u2010ligated side, the more severe the brain damage.<\/jats:p>","DOI":"10.1046\/j.1341-8076.2002.00063.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T07:38:39Z","timestamp":1047454719000},"page":"320-326","source":"Crossref","is-referenced-by-count":6,"title":["Cerebral blood flow distribution and hypoxic\u2013ischemic brain damage in newborn rats"],"prefix":"10.1111","volume":"28","author":[{"given":"Yi Xin","family":"Xia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sameshima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoaki","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Higo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsuyomu","family":"Ikenoue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11,23]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"217","volume-title":"Neurology of the Newborn","author":"Volpe JJ","year":"2001"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0887-8994(91)90060-X"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410110304"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198412000-00018"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)72459-8"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1992.tb00695.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410090206"},{"key":"e_1_2_5_9_2","first-page":"1","article-title":"Anoxic\u2010ischemic encephalopathy in rats","volume":"36","author":"Levine S","year":"1960","journal-title":"Am J Pathol"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315011"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410130507"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.1994.35"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.19.2.245"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.78.2.428"},{"key":"e_1_2_5_15_2","first-page":"H392","article-title":"Acute and chronic microsphere loss from canine ventricular myocardium","volume":"242","author":"Consigny PM","year":"1982","journal-title":"Am J Physiol"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(97)70139-X"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70625-3"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490330402"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(89)90383-7"},{"key":"e_1_2_5_20_2","volume-title":"A Stereotaxic Atlas of the Developing Rat Brain.","author":"Sherwood N","year":"1970"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1341-8076.2002.00042.x"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199005000-00007"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1985.tb10978.x"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(98)70156-5"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.10.2.147"},{"key":"e_1_2_5_26_2","first-page":"S143","article-title":"Hypoxic brain and heart injury thresholds in piglets","volume":"48","author":"De Courten\u2010Myers GM","year":"1989","journal-title":"Biomed Biochim Acta"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/000331976601701102"},{"key":"e_1_2_5_28_2","first-page":"26","article-title":"Adenosine in the control of the cerebral circulation","volume":"1","author":"Phillis JW","year":"1989","journal-title":"Cerebrovasc Brain Metab Rev"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(88)90192-6"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(79)80373-X"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1979.tb04430.x"},{"key":"e_1_2_5_32_2","first-page":"192","article-title":"Heterogeneity of hypoxic\u2010ischemic thresholds in experimental animals. 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