{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T15:39:31Z","timestamp":1791041971657,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":724,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    is a well\u2010studied plant pathogen, which has the unique ability to transfer DNA and protein into a number of eukaryotes. The DNA is integrated randomly into the plant genome where it is expressed, thereby leading to the disease crown gall. This system is a paradigm for the interaction of a number of plant and animal pathogens which transfer proteins into their host cells. In\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    , the tumour inducing (Ti) plasmid codes for the functions specifically required for the transfer process. These genes, termed virulence or\n                    <jats:italic>vir<\/jats:italic>\n                    genes, are activated by plant signal molecules acting through a two component regulatory system. A key structure coded by 11 genes of the\n                    <jats:italic>vir<\/jats:italic>\n                    B operon is a pilus, synthesized at 20\u00a0\u00b0C, but poorly at 25\u00a0\u00b0C. How this pilus functions in DNA and protein transfer is unclear, but homologous genes are found in many animal pathogens. In addition to Ti plasmid\u2010encoded\n                    <jats:italic>vir<\/jats:italic>\n                    genes, chromosomal virulence genes have also been identified. However, these mutations are often pleiotropic because they involve both the normal physiology of\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    as well as the metabolism of\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    when it is associated with plant cells. Based on 16S ribosomal RNA sequencing,\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    is closely related to the intracellular pathogen of animals,\n                    <jats:italic>Brucella<\/jats:italic>\n                    . Several chromosomal mutations of\n                    <jats:italic>Agrobacterium<\/jats:italic>\n                    required for virulence in plants are also required for invasion of animal host cells by\n                    <jats:italic>Brucella<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1046\/j.1364-3703.2000.00012.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:37:03Z","timestamp":1047461823000},"page":"87-90","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["DNA and protein transfer from bacteria to eukaryotes\u2014 the agrobacterium story"],"prefix":"10.1111","volume":"1","author":[{"given":"EugenE. W.","family":"Nester","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Molecular Plant Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1364-3703.2000.00012.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1364-3703.2000.00012.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1364-3703.2000.00012.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T18:05:37Z","timestamp":1762279537000},"score":1,"resource":{"primary":{"URL":"https:\/\/bsppjournals.onlinelibrary.wiley.com\/doi\/10.1046\/j.1364-3703.2000.00012.x"}},"subtitle":["The 18th Bateson Memorial Lecture"],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,1]]}},"alternative-id":["10.1046\/j.1364-3703.2000.00012.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1364-3703.2000.00012.x","archive":["Portico"],"relation":{},"ISSN":["1464-6722","1364-3703"],"issn-type":[{"value":"1464-6722","type":"print"},{"value":"1364-3703","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,1]]},"assertion":[{"value":"2001-12-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}