{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T13:06:34Z","timestamp":1760187994619},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":1242,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:p>This study aimed to demonstrate that sufficient Ph\u2010negative blood progenitors could be collected following administration of glycosylated rhG\u2010CSF (lenograstim) to patients with Philadelphia chromosome (Ph)\u2010positive chronic myeloid leukaemia (CML) who responded to recombinant alpha\u2010interferon (\u03b1\u2010IFN) (Ph\u2010positive marrow metaphases &lt;\u200335%). 23 patients received lenograstim (150\u2003\u03bcg\/m<jats:sup>2<\/jats:sup>) once daily for a median of 9\u2003d. Peak circulating numbers of white blood cells (36.4\u2003\u00d7\u200310<jats:sup>9<\/jats:sup>\/l), CD34<jats:sup>+<\/jats:sup> cells (24\/\u03bcl) and colony\u2010forming unit\u2010granulocyte\u2010macrophage (CFU\u2010GM; 1346.5\/ml) occurred at a median of day 8, day 8 and day 7, respectively. Two to six (median three) leukaphereses (LK) were performed from days 5 to 12. The median number of mononuclear cells (MNC), CD34<jats:sup>+<\/jats:sup> cells and CFU\u2010GM collected per patient was 7.35\u2003\u00d7\u200310<jats:sup>8<\/jats:sup>\/kg, 2.72\u2003\u00d7\u200310<jats:sup>6<\/jats:sup>\/kg and 10.23\u2003\u00d7\u200310<jats:sup>4<\/jats:sup>\/kg, respectively. 22\/23 patients had LK which contained either 10<jats:sup>4<\/jats:sup> CFU\u2010GM\/kg and\/or 10<jats:sup>6<\/jats:sup> CD34<jats:sup>+<\/jats:sup> cells\/kg; 47\u2003LK (from 20\/22 patients) were evaluable for cytogenetics. The median percentage of Ph\u2010positive cells was 0, and 43\/47 LK (91%) contained &lt;\u200335% Ph\u2010positive cells; 25 (53%) were entirely negative. Sixteen of 20 evaluable patients had one or more LK with &lt;\u200335% Ph\u2010positive cells, and 12 had at least one 100% Ph\u2010negative LK. Mobilization and collection of Ph\u2010negative cells were not influenced by the dose or duration of \u03b1\u2010IFN administration before (or during) lenograstim administration or by the quality of cytogenetic response (complete <jats:italic>v<\/jats:italic> major) during lenograstim administration. No significant side\u2010effects were observed. Thus, lenograstim administration can result in satisfactory Ph\u2010negative blood progenitor cell collection. Autologous transplantation of such cells may be used when indicated.<\/jats:p>","DOI":"10.1046\/j.1365-2141.1998.00821.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:03:20Z","timestamp":1047362600000},"page":"639-646","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Collection of Ph\u2010negative progenitor cells with granulocyte\u2010colony stimulating factor in patients with chronic myeloid leukaemia who respond to recombinant 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