{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T14:43:18Z","timestamp":1773153798429,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":541,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p>CD95 (Fas\/APO\u20101) expression and function and Bcl\u20102 expression, as well as spontaneous apoptosis <jats:italic>in vitro,<\/jats:italic> have been shown to be predictive markers for the <jats:italic>in vivo<\/jats:italic> response to chemotherapy in acute myeloid leukaemia (AML). To determine the clinical significance of apoptosis\u2010regulating factors in acute lymphoblastic leukaemia (ALL), we investigated cell samples of children with ALL who had been included in the German ALL Berlin\u2013Frankfurt\u2013M\u00fcnster (BFM) study using flow cytometry for constitutive expression levels of CD95 (<jats:italic>n<\/jats:italic>\u2003=\u2003110) and Bcl\u20102 (<jats:italic>n<\/jats:italic>\u2003=\u2003110). Furthermore, we determined the extent of spontaneous apoptosis <jats:italic>in vitro<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2003=\u2003102) and susceptibility to anti\u2010CD95\u2010induced apoptosis (CD95\u2010sensitivity) (<jats:italic>n<\/jats:italic>\u2003=\u200397). We correlated these findings with the functional activity of the multidrug resistance (MDR)\u2010associated P\u2010glycoprotein (P\u2010gp), as detected by the rhodamine123 efflux test, immunophenotype, cytogenetics and clinical data of the patients examined. Good responders to initial prednisone therapy (\u2018prednisone response\u2019) revealed significantly higher Bcl\u20102 expression levels [5\u00b74\u2003\u00b1\u20033\u00b74 relative fluorescence intensity (RFI), <jats:italic>n<\/jats:italic>\u2003=\u200368] than poor responders (3\u00b77\u2003\u00b1\u20032\u00b76 RFI, <jats:italic>n<\/jats:italic>\u2003=\u200342; <jats:italic>P<\/jats:italic>\u2003=\u20030\u00b7002). There was no significant correlation between the other investigated parameters and prednisone response. Moreover, neither the CD95 and Bcl\u20102 expression levels nor the extent of spontaneous apoptosis <jats:italic>in vitro<\/jats:italic>, CD95 sensitivity or P\u2010gp function were correlated with the response to induction chemotherapy or relapse rate, either for B\u2010cell precursor ALL or T\u2010cell ALL. No consistent pattern of change in CD95 (<jats:italic>n<\/jats:italic>\u2003=\u200310) and Bcl\u20102 expression (<jats:italic>n<\/jats:italic>\u2003=\u20039) was noted in cases studied at both initial diagnosis and relapse. In conclusion, our findings underline the different cell biological features of primary AML and ALL cells.<\/jats:p>","DOI":"10.1046\/j.1365-2141.2000.02147.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:25:46Z","timestamp":1047363946000},"page":"154-160","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Constitutive expression levels of CD95 and Bcl\u20102 as well as CD95 function and spontaneous apoptosis <i>in vitro<\/i> do not predict the response to induction chemotherapy and relapse rate in childhood acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"110","author":[{"given":"Christian","family":"Wuchter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonid","family":"Karawajew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Velia","family":"Ruppert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Schrappe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jochen","family":"Harbott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Ratei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernd","family":"D\u00f6rken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf\u2010Dieter","family":"Ludwig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1322"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0230940"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00827.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.10.3943"},{"key":"e_1_2_6_6_1","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. 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