{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:32:36Z","timestamp":1770823956988,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":479,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:p>Mantle\u2010cell lymphoma (MCL) has a poorer prognosis than other small B\u2010cell lymphomas, thus a definitive diagnosis is essential. The t(11;14)(q13;q32) associated with MCL juxtaposes portions of <jats:italic>CCND1<\/jats:italic> (11q13) and <jats:italic>IGH<\/jats:italic> (14q32), resulting in over\u2010expression of cyclin D1. In this study, a highly sensitive two\u2010colour fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) method was developed to detect t(11;14)(q13;q32) in nuclei isolated from paraffin\u2010embedded tissue. Twenty\u2010three MCLs, 13 normal controls and nine small B\u2010cell lymphomas other than MCL were studied by FISH. Each MCL had been previously investigated to detect genomic <jats:italic>IGH\u2013CCND1<\/jats:italic> fusion by polymerase chain reaction (PCR) using DNA extracted from frozen tissue. The <jats:italic>IGH\u2013CCND1<\/jats:italic> fusion detection rate in the MCLs was 96% by FISH compared with 35% by PCR. By FISH, one MCL and three small B\u2010cell lymphomas other than MCL harboured abnormalities involving only <jats:italic>IGH.<\/jats:italic> Less than 1% of cells showed false\u2010positive <jats:italic>IGH\u2013CCND1<\/jats:italic> fusion in normal specimens by FISH. Thus, this highly sensitive FISH assay is very useful in confirming the diagnosis of MCL, has wide applicability as it may be performed on both paraffin\u2010embedded and fresh tissue, and may also facilitate detection of translocations involving these loci in tumours other than MCL.<\/jats:p>","DOI":"10.1046\/j.1365-2141.2000.02303.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:28:31Z","timestamp":1047364111000},"page":"856-862","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":101,"title":["Diagnostic utility of fluorescence <i>in situ<\/i> hybridization in mantle\u2010cell lymphoma"],"prefix":"10.1111","volume":"110","author":[{"given":"E. D.","family":"Remstein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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